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Reference
Citation
Huet, F., Lu, J.T., Myrick, K.V., Baugh, L.R., Crosby, M.A., Gelbart, W.M. (2002). A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation.  Proc. Natl. Acad. Sci. U.S.A. 99(15): 9948--9953.
FlyBase ID
FBrf0151478
Publication Type
Research paper
Abstract

With the available eukaryotic genome sequences, there are predictions of thousands of previously uncharacterized genes without known function or available mutational variant. Thus, there is an urgent need for efficient genetic tools for genomewide phenotypic analysis. Here we describe such a tool: a deletion-generator technology that exploits properties of a double transposable element to produce molecularly defined deletions at high density and with high efficiency. This double element, called P[wHy], is composed of a "deleter" element hobo, bracketed by two genetic markers and inserted into a "carrier" P element. We have used this P[wHy] element in Drosophila melanogaster to generate sets of nested deletions of sufficient coverage to discriminate among every transcription unit within 60 kb of the starting insertion site. Because these two types of mobile elements, carrier and deleter, can be found in other species, our strategy should be applicable to phenotypic analysis in a variety of model organisms.

PubMed ID
PubMed Central ID
PMC126605 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Huet et al. deletions.
Huet and Gelbart, 2002.11, Huet et al. deletions. [FBrf0155386]

Note

Another arrow in the Drosophila quiver.
Kornberg, 2002, Proc. Natl. Acad. Sci. U.S.A. 99(15): 9607--9608 [FBrf0151476]

Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (5)
    Genes (36)
    Datasets (1)
    Molecular Constructs (1)
    Molecular Segments (3)
    Natural transposons (1)
    Insertions (596)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Transgenic Constructs (2)