| Citation |
Huet, F., Lu, J.T., Myrick, K.V., Baugh, L.R., Crosby, M.A., Gelbart, W.M. (2002). A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation. Proc. Natl. Acad. Sci. USA 99(15): 9948--9953. |
| PubMed Abstract |
With the available eukaryotic genome sequences, there are predictions of thousands of previously uncharacterized genes without
known function or available mutational variant. Thus, there is an urgent need for efficient genetic tools for genomewide phenotypic
analysis. Here we describe such a tool: a deletion-generator technology that exploits properties of a double transposable
element to produce molecularly defined deletions at high density and with high efficiency. This double element, called P[wHy],
is composed of a "deleter" element hobo, bracketed by two genetic markers and inserted into a "carrier" P element. We have
used this P[wHy] element in Drosophila melanogaster to generate sets of nested deletions of sufficient coverage to discriminate
among every transcription unit within 60 kb of the starting insertion site. Because these two types of mobile elements, carrier
and deleter, can be found in other species, our strategy should be applicable to phenotypic analysis in a variety of model
organisms.
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