Reference Report

Reference
Citation	Muller, J., Hart, C.M., Francis, N.J., Vargas, M.L., Sengupta, A., Wild, B., Miller, E.L., O'Connor, M.B., Kingston, R.E., Simon, J.A. (2002). Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell 111(2): 197--208. (Export to RIS)
FlyBase ID	FBrf0151815
Publication Type	Research paper
PubMed ID	12408864
PubMed Abstract	Polycomb group (PcG) proteins maintain transcriptional repression during development, likely by creating repressive chromatin states. The Extra Sex Combs (ESC) and Enhancer of Zeste [E(Z)] proteins are partners in an essential PcG complex, but its full composition and biochemical activities are not known. A SET domain in E(Z) suggests this complex might methylate histones. We purified an ESC-E(Z) complex from Drosophila embryos and found four major subunits: ESC, E(Z), NURF-55, and the PcG repressor, SU(Z)12. A recombinant complex reconstituted from these four subunits methylates lysine-27 of histone H3. Mutations in the E(Z) SET domain disrupt methyltransferase activity in vitro and HOX gene repression in vivo. These results identify E(Z) as a PcG protein with enzymatic activity and implicate histone methylation in PcG-mediated silencing.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (1)
	Df(1)w67c23
Alleles (12)
	E(z)⁶³ E(z)³²⁸ E(z)⁷³¹ E(z)¹⁰²⁵ E(z)²⁴³⁴ E(z)^c.hs E(z)^g.hs E(z)^H730K.hs E(z)^R699H.hs esc^T:Zzzz\FLAG esc^unspecified Su(z)12¹
Constructs (5)
	P{FLAG-esc} P{hs-E(z)^H730K} P{hs-E(z)^R699H} P{hs-E(z)cDNA} P{hs-E(z)genomic}
Genes (8)
	Abd-B Caf1 E(z) esc Rpd3 Su(z)12 T:Zzzz\FLAG Ubx
Polypeptides (1)
	Su(z)12⁺P900