Reference Report

Reference
Citation	Jackson, S.M., Berg, C.A. (2002). An A-kinase anchoring protein is required for Protein kinase A regulatory subunit localization and morphology of actin structures during oogenesis in Drosophila. Development 129(19): 4423--4433. (Export to RIS)
FlyBase ID	FBrf0151923
Publication Type	Research paper
PubMed ID	12223401
PubMed Abstract	Protein kinase A (PKA) holoenzyme is anchored to specific subcellular regions by interactions between regulatory subunits (Pka-R) and A-kinase anchoring proteins (AKAPs). We examine the functional importance of PKA anchoring during Drosophila oogenesis by analyzing membrane integrity and actin structures in mutants with disruptions in Akap200, an AKAP. In wild-type ovaries, Pka-RII and Akap200 localized to membranes and to the outer rim of ring canals, actin-rich structures that connect germline cells. In Akap200 mutant ovaries, Pka-RII membrane localization decreased, leading to a destabilization of membrane structures and the formation of binucleate nurse cells. Defects in membrane integrity could be mimicked by expressing a constitutively active PKA catalytic subunit (Pka-C) throughout germline cells. Unexpectedly, nurse cells in Akap200 mutant ovaries also had enlarged, thin ring canals. In contrast, overexpressing Akap200 in the germline resulted in thicker, smaller ring canals. To investigate the role of Akap200 in regulating ring canal growth, we examined genetic interactions with other genes that are known to regulate ring canal morphology. Akap200 mutations suppressed the small ring canal phenotype produced by Src64B mutants, linking Akap200 with the non-receptor tyrosine kinase pathway. Together, these results provide the first evidence that PKA localization is required for morphogenesis of actin structures in an intact organism.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2L)N22-14
Alleles (18)
	Akap200^EP2254 Akap200^ix1 Akap200^ix2 Akap200^ix4 Akap200^k07118a Akap200^Δ7 l(2)k07118b^k07118b Mmus\Pkaca^mC.Scer\UAS Ocun\PKI^{M.Scer\UAS.T:Zzzz\FLAG} Ocun\PKI^{Scer\UAS.T:Zzzz\FLAG} Pka-C1^Scer\UAS.cKa Scer\GAL4^nos.PG Scer\GAL4^{nos.UTR.T:Hsim\VP16} Src64B^PI w^+mC w^mC.ix1 w^mC.ix2 w^mC.ix4
Constructs (10)
	P{GAL4::VP16-nos.UTR} P{GAL4-nos.NGT} P{lacW} P{lacW-Δix1} P{lacW-Δix2} P{lacW-Δix4} P{UAS-Ocun\PKI.F} P{UAS-Ocun\PKI.M.F} P{UAS-PKA.mC} P{UAS-Pka-C1.K}
Genes (14)
	Akap200 cher hts kel l(2)k07118b Mmus\Pkaca Ocun\PKI Pka-C1 Pka-R2 Scer\GAL4 Src64B T:Hsim\VP16 T:Zzzz\FLAG w
Insertions (5)
	P{EP}Akap200^EP2254 P{lacW}Akap200^k07118a P{lacW-Δix1}Akap200^ix1 P{lacW-Δix2}Akap200^ix2 P{lacW-Δix4}Akap200^ix4