Reference Report

Reference
Citation	Doronkin, S., Djagaeva, I., Beckendorf, S.K. (2002). CSN5/Jab1 mutations affect axis formation in the Drosophila oocyte by activating a meiotic checkpoint. Development 129(21): 5053--5064. (Export to RIS)
FlyBase ID	FBrf0151933
Publication Type	Research paper
PubMed ID	12397113
PubMed Abstract	The COP9 signalosome (CSN) is linked to signaling pathways and ubiquitin-dependent protein degradation in yeast, plant and mammalian cells, but its roles in Drosophila development are just beginning to be understood. We show that during oogenesis CSN5/JAB1, one subunit of the CSN, is required for meiotic progression and for establishment of both the AP and DV axes of the Drosophila oocyte. The EGFR ligand Gurken is essential for both axes, and our results show that CSN5 mutations block the accumulation of Gurken protein in the oocyte. CSN5 mutations also cause the modification of Vasa, which is known to be required for Gurken translation. This CSN5 phenotype - defective axis formation, reduced Gurken accumulation and modification of Vasa - is very similar to the phenotype of the spindle-class genes that are required for the repair of meiotic recombination-induced, DNA double-strand breaks. When these breaks are not repaired, a DNA damage checkpoint mediated by mei-41 is activated. Accordingly, the CSN5 phenotype is suppressed by mutations in mei-41 or by mutations in mei-W68, which is required for double strand break formation. These results suggest that, like the spindle-class genes, CSN5 regulates axis formation by checkpoint-dependent, translational control of Gurken. They also reveal a link between DNA repair, axis formation and the COP9 signalosome, a protein complex that acts in multiple signaling pathways by regulating protein stability.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (22)
	CSN5^ex9 CSN5^ex13 CSN5^ex15 CSN5^ex21 CSN5^ex27 CSN5^ex35 CSN5^L4032 Ecol\lacZ⁶³⁵⁶ Ecol\lacZ^kek1-15A6 Ecol\lacZ^{P\T.T:Khc-606} Ecol\lacZ^slbo-01310 Egfr^E1 Egfr^f2 Egfr^t1 grk^2B6 grk³ grk^HF mei-41^D1 mei-41^D3 mei-41^unspecified mei-W68¹ mei-W68^k05603
Constructs (1)
	P{KZ.TRAP}
Genes (21)
	bcd capu chic CSN5 dpp Ecol\lacZ Egfr enc fs(1)K10 grk mei-41 mei-W68 osk Ras85D rho rl spir sqd T:Khc-606 twi vas
Insertions (4)
	P{lacW}CSN5^L4032 P{lArB}kek1^15A6 P{PZ}6356 P{PZ}slbo⁰¹³¹⁰