|Citation||Jauch, E., Melzig, J., Brkulj, M., Raabe, T. (2002). In vivo functional analysis of Drosophila protein kinase casein kinase 2 (CK2) beta-subunit. Gene 298(1): 29--39. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||Protein casein kinase 2 (CK2) is a heterotetramer composed of two catalytic (alpha) and two regulatory (beta) subunits. In Drosophila melanogaster, four genes encoding for one CK2alpha (DmCK2alpha) and three CK2beta (DmCK2beta, DmCK2beta' and DmCK2betates) subunits have been identified. Here, we have focused on the role of DmCK2beta. Evaluation of DNA complementary to RNA sequences and Western blot analysis revealed a complex pattern of DmCK2beta transcription and the existence of at least three distinct DmCK2beta isoforms in adult flies. The phenotype of the viable DmCK2beta mutation mushroom bodies undersized(P1) (DmCK2beta(mbuP1)) described in this study implicates a role for DmCK2beta in cell proliferation or cell survival during brain development. The isolation of a complete loss of function allele of DmCK2beta allowed us to assay the importance of various structural domains for the in vivo function of DmCK2beta. Expression of in vitro mutagenised DmCK2beta transgenes in a DmCK2beta null mutant background demonstrated an absolute requirement of the beta/beta dimerisation motif for CK2beta function, whereas removal of all N-terminal phosphorylation sites of DmCK2beta resulted in reduced viability. In contrast, a mutation in the predicted destruction box motif did not interfere with DmCK2beta function.|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Language of Publication||English|
|Additional Languages of Abstract|
|Also Published As|
|Data from Reference|