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Reference
Citation	Salvaterra, P.M., Kitamoto, T. (2001). Drosophila cholinergic neurons and processes visualized with Gal4/UAS-GFP. Brain Res. Gene Expression Patterns 1(1): 73--82.
FlyBase ID	FBrf0151994
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	15018821
PubMed Abstract	Using 7.4 kb of 5' flanking DNA from the Drosophila cholinergic gene locus to drive Gal4 expression we can visualize essentially all cholinergic neurons and neuropiles after genetic recombination with a UAS-GFP (S65T) reporter gene. In contrast to previous methods somata and neuropiles can be observed in the same samples. Fluorescence intensity is strong enough to allow observations in live animals at all developmental stages. Three-dimensional reconstructions made from confocal sections of whole-mount preparations reveal the extensive cholinergic connections among various regions of the nervous system.
Biosis	2002.505338
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Language of publication	English
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Abbreviation	Brain Res. Gene Expression Patterns
Title	Brain Research. Gene Expression Patterns
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Volume range	1
Year range	2001
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Place of publication	Amsterdam
Language of publication	English
ISBN/ISSN	1567-133X
CODEN	GEPEAD
Data from Reference
Alleles (3)
	Avic\GFP^{S65T.Scer\UAS} Scer\GAL4^Cha.7.4 w^+mC
Constructs (2)
	P{Cha-GAL4.7.4} P{UAS-GFP.S65T}
Genes (4)
	Avic\GFP Cha Scer\GAL4 w
Insertions (1)
	P{Cha-GAL4.7.4}19B