Reference Report

Reference
Citation	Dauwalder, B., Tsujimoto, S., Moss, J., Mattox, W. (2002). The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior. Genes Dev. 16(22): 2879--2892. (Export to RIS)
FlyBase ID	FBrf0152012
Publication Type	Research paper
PubMed ID	12435630
PubMed Abstract	The Drosophila somatic sex-determination regulatory pathway has been well studied, but little is known about the target genes that it ultimately controls. In a differential screen for sex-specific transcripts expressed in fly heads, we identified a highly male-enriched transcript encoding Takeout, a protein related to a superfamily of factors that bind small lipophilic molecules. We show that sex-specific takeout transcripts derive from fat body tissue closely associated with the adult brain and are dependent on the sex determination genes doublesex (dsx) and fruitless (fru). The male-specific Doublesex and Fruitless proteins together activate Takeout expression, whereas the female-specific Doublesex protein represses takeout independently of Fru. When cells that normally express takeout are feminized by expression of the Transformer-F protein, male courtship behavior is dramatically reduced, suggesting that male identity in these cells is necessary for behavior. A loss-of-function mutation in the takeout gene reduces male courtship and synergizes with fruitless mutations, suggesting that takeout plays a redundant role with other fru-dependent factors involved in male mating behavior. Comparison of Takeout sequences to the Drosophila genome reveals a family of 20 related secreted factors. Expression analysis of a subset of these genes suggests that the takeout gene family encodes multiple factors with sex-specific functions.
DOI
Related Publication(s)
Review	Fat bodies and sex. Anonymous, 2002, Science 298(5599): 1683 [FBrf0155373]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (4)
	C(1;Y)1 C(1)RM Tp(3;Y)A81 Tp(3;Y)L58
Alleles (11)
	dsx¹ dsx^S fru³ fru⁴ Scer\GAL4^to.PD to¹ to^+t6.4 tra2^PM6 tra2^PM7 tra^Scer\UAS.cFa w^+mC
Constructs (2)
	P{to-GAL4.D} P{UAS-tra.F}
Genes (28)
	CG1124 CG2016 CG2650 CG5867 CG5945 CG7079 CG10264 CG10407 CG11852 CG11854 CG13618 CG14258 CG14259 CG14457 CG16820 CG17189 CG17279 CG31189 CG31207 dsx eater fru olf413 Scer\GAL4 to tra tra2 w
Insertions (1)
	P{PZ}fru⁴