Reference Report

Reference
Citation	Tseng, A.S., Hariharan, I.K. (2002). An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. Genetics 162(1): 229--243. (Export to RIS)
FlyBase ID	FBrf0152035
Publication Type	Research paper
PubMed ID	12242236
PubMed Abstract	We screened for genes that, when overexpressed in the proliferating cells of the eye imaginal disc, result in a reduction in the size of the adult eye. After crossing the collection of 2296 EP lines to the ey-GAL4 driver, we identified 46 lines, corresponding to insertions in 32 different loci, that elicited a small eye phenotype. These lines were classified further by testing for an effect in postmitotic cells using the sev-GAL4 driver, by testing for an effect in the wing using en-GAL4, and by testing for the ability of overexpression of cycE to rescue the small eye phenotype. EP lines identified in the screen encompass known regulators of eye development including hh and dpp, known genes that have not been studied previously with respect to eye development, as well as 19 novel ORFs. Lines with insertions near INCENP, elB, and CG11518 were characterized in more detail with respect to changes in growth, cell-cycle phasing, and doubling times that were elicited by overexpression. RNAi-induced phenotypes were also analyzed in SL2 cells. Thus overexpression screens can be combined with RNAi experiments to identify and characterize new regulators of growth and cell proliferation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (70)
	aop^EP598 aop^EP2500 BacA\p35^Scer\UAS.cHa bol^EP3659 CG6701^EP2054 CG9650^EP1617 CG9932^EP2317 CG12084^EP3673 CG42709^EP3622 ckd^EP714 ckd^EP3041 ckd^EP3139 ckd^EP3560 ckd^EP3689 ckd^EP3718 Cul-5^EP3390 cv^EP1349 CycE^Scer\UAS.cLa dap^{Scer\UAS.cdNa} dbr^EP456 dpp^EP2232 Eip78C^EP3468 elB^dsRNA.cTa elB^EP965 elB^EP2039 EP1595^EP1595 EP2419^EP2419 EP3617^EP3617 esg^EP633 esg^EP684 esg^EP2009 esg^EP2408 fng^EP3082 hh^EP3521 Hr4^EP1232 Hr39^EP2490 Hsap\CDKN1A^Scer\UAS.cHa Incenp^dsRNA.cTa Incenp^EP2340 Incenp^Scer\UAS.cTa kibra^EP666 kibra^EP747 Kr-h1^EP2289 l(3)L0539^EP3083 l(3)L0539^EP3523 mask^EP601 nej^EP1149 nej^EP1179 NFAT^EP1335 NFAT^EP1390 NFAT^EP1508 noc^dsRNA.cTa pbl^EP3415 Pka-R2^EP2162 pygo^dsRNA.cTa pygo^EP1076 qsm^EP2586 Rac2^EP3118 Rbf^Scer\UAS.cDa rho^EP3704 Scer\GAL4^Act5C.PP Scer\GAL4^en-e16E Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^hs.2sev Scer\GAL4^prd.RG1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} shn^EP2359 Traf4^EP578 YT521-B^EP3725
Constructs (12)
	P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{GAL4-ey.H} P{GAL4-Hsp70.sev} P{GAL4-ninaE.GMR} P{GAL4-prd.F} P{UAS-CycE.L} P{UAS-dap.dN} P{UAS-Incenp.T} P{UAS-p21.H} P{UAS-p35.H} P{UAS-Rbf.D}
Genes (50)
	aop BacA\p35 bol CG6701 CG9650 CG9932 CG11068 CG11873 CG12084 CG12362 CG32795 CG42709 ckd cv CycE dap dbr dpp Eip78C elB EP1595 EP2419 EP3390 EP3617 esg fng hh Hr4 Hr39 Hsap\CDKN1A Incenp kibra Kr-h1 l(3)L0539 mask nej NFAT noc pbl Pka-R2 pygo qsm Rac2 RapGAP1 Rbf rho Scer\GAL4 shn Traf4 YT521-B
Insertions (54)
	P{en2.4-GAL4}e16E P{EP}aop^EP598 P{EP}aop^EP2500 P{EP}bol^EP3659 P{EP}CG6701^EP2054 P{EP}CG9650^EP1617 P{EP}CG9932^EP2317 P{EP}CG12084^EP3673 P{EP}CG42709^EP3622 P{EP}ckd^EP714 P{EP}ckd^EP3041 P{EP}ckd^EP3139 P{EP}ckd^EP3560 P{EP}ckd^EP3689 P{EP}ckd^EP3718 P{EP}Cul-5^EP3390 P{EP}cv^EP1349 P{EP}dbr^EP456 P{EP}dpp^EP2232 P{EP}Eip78C^EP3468 P{EP}elB^EP965 P{EP}elB^EP2039 P{EP}EP1595^EP1595 P{EP}EP2419^EP2419 P{EP}EP3617^EP3617 P{EP}esg^EP633 P{EP}esg^EP684 P{EP}esg^EP2009 P{EP}esg^EP2408 P{EP}fng^EP3082 P{EP}hh^EP3521 P{EP}Hr4^EP1232 P{EP}Hr39^EP2490 P{EP}Incenp^EP2340 P{EP}kibra^EP666 P{EP}kibra^EP747 P{EP}Kr-h1^EP2289 P{EP}l(3)L0539^EP3083 P{EP}l(3)L0539^EP3523 P{EP}mask^EP601 P{EP}nej^EP1149 P{EP}nej^EP1179 P{EP}NFAT^EP1335 P{EP}NFAT^EP1390 P{EP}NFAT^EP1508 P{EP}pbl^EP3415 P{EP}Pka-R2^EP2162 P{EP}pygo^EP1076 P{EP}qsm^EP2586 P{EP}Rac2^EP3118 P{EP}rho^EP3704 P{EP}shn^EP2359 P{EP}Traf4^EP578 P{EP}YT521-B^EP3725