Reference Report

Reference
Citation	Vilinsky, I., Stewart, B.A., Drummond, J., Robinson, I., Deitcher, D.L. (2002). A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission. Genetics 162(1): 259--271. (Export to RIS)
FlyBase ID	FBrf0152037
Publication Type	Research paper
External Crossreferences
PubMed ID	12242238
PubMed Abstract	The synaptic protein SNAP-25 is an important component of the neurotransmitter release machinery, although its precise function is still unknown. Genetic analysis of other synaptic proteins has yielded valuable information on their role in synaptic transmission. In this study, we performed a mutagenesis screen to identify new SNAP-25 alleles that fail to complement our previously isolated recessive temperature-sensitive allele of SNAP-25, SNAP-25(ts). In a screen of 100,000 flies, 26 F(1) progeny failed to complement SNAP-25(ts) and 21 of these were found to be null alleles of SNAP-25. These null alleles die at the pharate adult stage and electroretinogram recordings of these animals reveal that synaptic transmission is blocked. At the third instar larval stage, SNAP-25 nulls exhibit nearly normal neurotransmitter release at the neuromuscular junction. This is surprising since SNAP-25(ts) larvae exhibit a much stronger synaptic phenotype. Our evidence indicates that a related protein, SNAP-24, can substitute for SNAP-25 at the larval stage in SNAP-25 nulls. However, if a wild-type or mutant form of SNAP-25 is present, then SNAP-24 does not appear to take part in neurotransmitter release at the larval NMJ. These results suggest that the apparent redundancy between SNAP-25 and SNAP-24 is due to inappropriate genetic substitution.
BIOSIS ID	2002.572280
Zoological Record ID
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
ISBN
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Editors
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (5)
	Df(3L)1-16 Df(3L)Snap25³ Df(3L)Snap25³³ Df(3L)Snap25⁵³ Df(3L)Snap25¹⁵³
Alleles (30)
	Snap24^hs.PN Snap25^1-16-0 Snap25³ Snap25¹⁴ Snap25¹⁶ Snap25²² Snap25²⁷ Snap25³³ Snap25³⁴ Snap25⁴² Snap25⁴⁴ Snap25⁴⁶ Snap25⁴⁸ Snap25⁵² Snap25⁵³ Snap25⁷⁰ Snap25⁸⁷ Snap25⁹³ Snap25¹¹⁴ Snap25¹²⁴ Snap25¹²⁵ Snap25¹²⁶ Snap25¹³² Snap25¹³³ Snap25¹⁴⁷ Snap25¹⁵³ Snap25¹⁶⁴ Snap25⁶⁹⁵ Snap25^n-syb.PR Snap25^ts
Constructs (2)
	P{hs-Snap24.N} P{n-syb-Snap25.R}
Genes (4)
	Snap24 Snap25 Syt1 Syx1A