Reference Report

Reference
Citation	Stapleton, M., Liao, G., Brokstein, P., Hong, L., Carninci, P., Shiraki, T., Hayashizaki, Y., Champe, M., Pacleb, J., Wan, K., Yu, C., Carlson, J., George, R., Celniker, S., Rubin, G.M. (2002). The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. Genome Res. 12(8): 1294--1300. (Export to RIS)
FlyBase ID	FBrf0152058
Publication Type	Research paper
PubMed ID	12176937
PubMed Abstract	Collections of full-length nonredundant cDNA clones are critical reagents for functional genomics. The first step toward these resources is the generation and single-pass sequencing of cDNA libraries that contain a high proportion of full-length clones. The first release of the Drosophila Gene Collection Release 1 (DGCr1) was produced from six libraries representing various tissues, developmental stages, and the cultured S2 cell line. Nearly 80,000 random 5' expressed sequence tags (5' expressed sequence tags [ESTs]from these libraries were collapsed into a nonredundant set of 5849 cDNAs, corresponding to ~40% of the 13,474 predicted genes in Drosophila. To obtain cDNA clones representing the remaining genes, we have generated an additional 157,835 5' ESTs from two previously existing and three new libraries. One new library is derived from adult testis, a tissue we previously did not exploit for gene discovery; two new cap-trapped normalized libraries are derived from 0-22-h embryos and adult heads. Taking advantage of the annotated D. melanogaster genome sequence, we clustered the ESTs by aligning them to the genome. Clusters that overlap genes not already represented by cDNA clones in the DGCr1 were analyzed further, and putative full-length clones were selected for inclusion in the new DGC. This second release of the DGC (DGCr2) contains 5061 additional clones, extending the collection to 10,910 cDNAs representing >70% of the predicted genes in Drosophila.
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Related Publication(s)
Personal communication to FlyBase	ESTs generated by the Drosophila Gene Collection Project after the year 2000. Celniker, 2006.2.21, ESTs generated by the Drosophila Gene Collection Project after the year 2000. [FBrf0188760]
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genome Res.
Title	Genome Research
Publication Year	1995-
ISBN/ISSN	1088-9051
Data from Reference
Constructs (1)
	pOTB7
Genes (1)
	Cctγ
Libraries/Collections (3)
	AT_cDNA RE_cDNA RH_cDNA