Reference Report

Reference
Citation	Fulga, T.A., Rorth, P. (2002). Invasive cell migration is initiated by guided growth of long cellular extensions. Nat. Cell Biol. 4(9): 715--719. (Export to RIS)
FlyBase ID	FBrf0152247
Publication Type	Research paper
PubMed ID	12198500
PubMed Abstract	The migration of border cells during Drosophila melanogaster oogenesis is a simple and powerful system for studying invasive cell migration in vivo. Border cells are somatic cells that delaminate from the follicular epithelium of an egg chamber and invade the germ line cluster. They migrate between the nurse cells to reach the oocyte, using DE-cadherin for adhesion to the substratum. Border cells take approximately 6 h to migrate a distance of 100 microm. The migration is guided by EGFR (epidermal growth factor receptor) and PVR (platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) receptor). Here, we show that a single long cellular extension (LCE), several cell diameters in length, is formed at the initiation of migration. The LCE may function as a 'pathfinder' in response to guidance cues. LCE growth requires directional guidance signals and specific adhesion to the substratum. Interference with actin-myosin interactions allows continued LCE growth while preventing translocation of the cell bodies. We discuss similarities between LCEs and axons and the use of LCE-like structures as a general mechanism for initiating invasive migration in vivo.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Alleles (12)
	Act5C^{slbo.T:Avic\GFP-EGFP} Ecol\lacZ^slbo.FRT Egfr^DN.Scer\UAS Pi3K92E^{D954A.Scer\UAS.T:Hsap\MYC} Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} Pvf1^EPg11235 Pvr^DN.Scer\UAS Scer\GAL4^slbo.2.6 shg¹ sqh^AX3 T:Avic\GFP^EGFP vn^EPg35521
Constructs (7)
	P{Dp110^D954A} P{Dp110-CAAX} P{GAL4-slbo.2.6} P{slbo(FRT.lacZ)} P{slbo-EGFP::Act5C} P{UAS-Egfr.DN} P{UAS-Pvr.DN}
Genes (13)
	Act5C Ecol\lacZ Egfr Pi3K92E Pvf1 Pvr Scer\GAL4 shg sqh T:Avic\GFP T:Hsap\CAAX T:Hsap\MYC vn
Insertions (2)
	P{EPg}Pvf1^EPg11235 P{EPg}vn^EPg35521