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Wheeler, J.C., van der Zwan, C., Xu, X., Swantek, D., Tracey, W.D., Gergen, J.P. (2002). Distinct in vivo requirements for establishment versus maintenance of transcriptional repression.  Nat. Genet. 32(1): 206--210.
FlyBase ID
FBrf0152256
Publication Type
Research paper
Abstract

Low-level ectopic expression of the Runt transcription factor blocks activation of the Drosophila melanogaster segmentation gene engrailed (en) in odd-numbered parasegments and is associated with a lethal phenotype. Here we show, by using a genetic screen for maternal factors that contribute in a dose-dependent fashion to Runt-mediated repression, that there are two distinct steps in the repression of en by Runt. The initial establishment of repression is sensitive to the dosage of the zinc-finger transcription factor Tramtrack. By contrast, the co-repressor proteins Groucho and dCtBP, and the histone deacetylase Rpd3, do not affect establishment but instead maintain repression after the blastoderm stage. The distinction between establishment and maintenance is confirmed by experiments with Runt derivatives that are impaired specifically for either co-repressor interaction or DNA binding. Other transcription factors can also establish repression in Rpd3-deficient embryos, which indicates that the distinction between establishment and maintenance may be a general feature of eukaryotic transcriptional repression.

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Note

Stopping transcription in its tracks.
Jennings, 2002, Nat. Genet. 32(1): 87--88 [FBrf0152258]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Genet.
    Title
    Nature Genetics
    Publication Year
    1992-
    ISBN/ISSN
    1061-4036 1546-1718
    Data From Reference
    Aberrations (4)
    Alleles (18)
    Genes (11)
    Insertions (2)
    Experimental Tools (2)
    Transgenic Constructs (7)