From kcook@XXXX Tue Dec 17 00:32:17 2002 To: flybase-updates@XXXX Subject: Isolation and characterization of Df(2R)BSC26 Isolation and characterization of Df(2R)BSC26 Megan Deal-Herr and Kevin Cook Bloomington Drosophila Stock Center, Indiana University Df(2R)BSC26 was isolated as a P transposase-induced male recombination event involving P{lacW}corak08713 and P{PZ}hts01103. The deletion was isolated as a cn+-bw+recombinant chromosome from the cross cn1 bw1 females X cn1 P{PZ}hts01103/ P{lacW}corak08713 bw1 sp1; TMS, Sb1 P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints 56C4;56D6-10. The deficiency chromosome retains the miniwhite marker from P{lacW}corak08713. Df(2R)BSC26 failed to complement wblE4. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX