Reference Report

Reference
Citation	Ang, L.H., Kim, J., Stepensky, V., Hing, H. (2003). Dock and Pak regulate olfactory axon pathfinding in Drosophila. Development 130(7): 1307--1316. (Export to RIS)
FlyBase ID	FBrf0155701
Publication Type	Research paper
PubMed ID	12588847
PubMed Abstract	The convergence of olfactory axons expressing particular odorant receptor (Or) genes on spatially invariant glomeruli in the brain is one of the most dramatic examples of precise axon targeting in developmental neurobiology. The cellular and molecular mechanisms by which olfactory axons pathfind to their targets are poorly understood. We report here that the SH2/SH3 adapter Dock and the serine/threonine kinase Pak are necessary for the precise guidance of olfactory axons. Using antibody localization, mosaic analyses and cell-type specific rescue, we observed that Dock and Pak are expressed in olfactory axons and function autonomously in olfactory neurons to regulate the precise wiring of the olfactory map. Detailed analyses of the mutant phenotypes in whole mutants and in small multicellular clones indicate that Dock and Pak do not control olfactory neuron (ON) differentiation, but specifically regulate multiple aspects of axon trajectories to guide them to their cognate glomeruli. Structure/function studies show that Dock and Pak form a signaling pathway that mediates the response of olfactory axons to guidance cues in the developing antennal lobe (AL). Our findings therefore identify a central signaling module that is used by ONs to project to their cognate glomeruli.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (23)
	Avic\GFP^{Scer\UAS.T:Hsap\Myr2} dock⁰⁴⁷²³ dock^{R336Q.Scer\UAS} dock^Scer\UAS.cRa dock^{W48K.Scer\UAS} dock^{W151K.Scer\UAS} dock^{W225K.Scer\UAS} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} n-syb^{Scer\UAS.cEa.T:Avic\GFP} Pak⁴ Pak⁶ Pak¹¹ Pak¹⁶ Pak^{GMR.T:Myr-Src64B} Pak^{Scer\UAS.T:Hsap\MYC} Pak^{Scer\UAS.T:Myr-Src64B} Scer\FLP1^ey.PN Scer\GAL4^Dll-981 Scer\GAL4^GH146 Scer\GAL4^Or22a.7.717 Scer\GAL4^Or47a.8.239 Scer\GAL4^Or47b.7.467 Scer\GAL4^SG18.1
Constructs (14)
	P{ey-FLP.N} P{Or22a-GAL4.7.717} P{Or47a-GAL4.8.239} P{Or47b-GAL4.7.467} P{UAS-dock.R336Q} P{UAS-dock.R} P{UAS-dock.W48K} P{UAS-dock.W151K} P{UAS-dock.W225K} P{UAS-gapGFP} P{UAS-mCD8::GFP.L} P{UAS-n-syb-GFP.E} P{UAS-Pak.MYC} P{UAS-Pak.myr}
Genes (17)
	Avic\GFP dock elav futsch lz Mmus\Cd8a n-syb Or22a Or47a Or47b Pak Scer\FLP1 Scer\GAL4 T:Avic\GFP T:Hsap\MYC T:Hsap\Myr2 T:Myr-Src64B
Insertions (2)
	P{GawB}GH146 P{GawB}SG18.1
Transcripts (4)
	Scer\GAL4^Or22a.7.717RA Scer\GAL4^Or47a.8.239RA Scer\GAL4^Or47b.7.467RA Scer\GAL4^SG18.1RA