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Citation	Lunde, K., Trimble, J.L., Guichard, A., Guss, K.A., Nauber, U., Bier, E. (2003). Activation of the knirps locus links patterning to morphogenesis of the second wing vein in Drosophila. Development 130(2): 235--248.
FlyBase ID	FBrf0155705
Type of publication	Research paper
Offprint Available	No
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PubMed ID	12466192
PubMed Abstract	The adjacent knirps (kni) and knirps-related (knrl) genes encode functionally related zinc finger transcription factors that collaborate to initiate development of the second longitudinal wing vein (L2). kni and knrl are expressed in the third instar larval wing disc in a narrow stripe of cells just anterior to the broad central zone of cells expressing high levels of the related spalt genes. Here, we identify a 1.4 kb cis-acting enhancer element from the kni locus that faithfully directs gene expression in the L2 primordium. We find that three independent ri alleles have alterations mapping within the L2-enhancer element and show that two of these observed lesions eliminate the ability of the enhancer element to direct gene expression in the L2 primordium. The L2 enhancer can be subdivided into distinct activation and repression domains. The activation domain mediates the combined action of the general wing activator Scalloped and a putative locally provided factor, the activity of which is abrogated by a single nucleotide alteration in the ri(53j) mutant. We also find that misexpression of genes in L2 that are normally expressed in veins other than L2 results in abnormal L2 development. These experiments provide a mechanistic basis for understanding how kni and knrl link AP patterning to morphogenesis of the L2 vein by orchestrating the expression of a selective subset of vein-promoting genes in the L2 primordium.
Biosis	2003.106667
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Language of publication	English
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Abbreviation	Development
Title	Development
Authors
Volume range	99-
Year range	1987-
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Place of publication	Cambridge
Language of publication	English
ISBN/ISSN	0950-1991
CODEN	DEVPED
Data from Reference
Aberrations (2)
	Df(3L)kni-FC82 Df(3L)kni^ri-XT2
Alleles (38)
	CG13252^53j Dl^Scer\UAS.cUa Ecol\lacZ^EcoRI.F Ecol\lacZ^EcoRI.Q Ecol\lacZ^EcoRI.R Ecol\lacZ^EcoRI.S Ecol\lacZ^P\T.PC Ecol\lacZ^kni.E Ecol\lacZ^{kni.EC-3Sdmut} Ecol\lacZ^kni.EC Ecol\lacZ^kni.ES Ecol\lacZ^{kni.EX.ri-53j} Ecol\lacZ^kni.EX Ecol\lacZ^kni.EΔri-1 S^Scer\UAS.cGb Scer\GAL4^Bx-MS1096 Scer\GAL4^CY2 Scer\GAL4^kni.L2 T:λ\cI^DD ara^Scer\UAS.cGa btl::Egfr^{Scer\UAS.T:λ\cI-DD} caup^{Scer\UAS.cdCa} eg^Scer\UAS.cDa kni^Scer\UAS.cLa kni^ri-1 kni^ri-53j kni^ri-92f kni^ri-M3 kni^ri-XT2 kni^riM knrl^Scer\UAS.cLa rho^Scer\UAS.cBa sc^Scer\UAS.cCa sd^58d spi^s.Scer\UAS vn^Scer\UAS.cSa w^+mC wg^Scer\UAS.cUa
Constructs (25)
	P{EcoRI.F-lacZ} P{EcoRI.Q-lacZ} P{EcoRI.R-lacZ} P{EcoRI.S-lacZ} P{UAS-Dl.U} P{UAS-Egfr.λtop} P{UAS-Star.G} P{UAS-ara.G} P{UAS-caup.dC} P{UAS-eg.D} P{UAS-kni.L} P{UAS-knrl.L} P{UAS-sc.C} P{UAS-spi.s} P{UAS-ve.B} P{UAS-vn.S} P{UAS-wg.U} P{kni-GAL4.L2} P{kni-lacZ.EC-3Sdmut} P{kni-lacZ.EC} P{kni-lacZ.ES} P{kni-lacZ.EX(ri^53j)} P{kni-lacZ.EX} P{kni-lacZ.E} P{kni-lacZ.EΔri¹}
Genes (20)
	CG13252 Dl Ecol\lacZ S Scer\GAL4 T:λ\cI ara btl::Egfr caup eg kni knrl rho salm sc sd spi vn w wg
Insertions (2)
	P{GawB}Bx^MS1096 P{GawB}CY2