Reference Report

Reference
Citation	Butler, M.J., Jacobsen, T.L., Cain, D.M., Jarman, M.G., Hubank, M., Whittle, J.R., Phillips, R., Simcox, A. (2003). Discovery of genes with highly restricted expression patterns in the Drosophila wing disc using DNA oligonucleotide microarrays. Development 130(4): 659--670. (Export to RIS)
FlyBase ID	FBrf0155718
Publication Type	Research paper
PubMed ID	12505997
PubMed Abstract	The Drosophila wing disc is divided along the proximal-distal axis into regions giving rise to the body wall (proximal), wing hinge (central) and wing blade (distal). We applied DNA microarray analysis to discover genes with potential roles in the development of these regions. We identified a set of 94 transcripts enriched (two fold or greater) in the body wall and 56 transcripts enriched in the wing/hinge region. Transcripts that are known to have highly restricted expression patterns, such as pannier, twist and Bar-H1 (body wall) and knot, nubbin and Distal-less (wing/hinge), showed strong differential expression on the arrays. In situ hybridization for 50 previously uncharacterized genes similarly revealed that transcript enrichment identified by the array analysis was consistent with the observed spatial expression. There was a broad spectrum of patterns, in some cases suggesting that the genes could be targets of known signaling pathways. We show that three of these genes respond to wingless signaling. We also discovered genes likely to play specific roles in tracheal and myoblast cell types, as these cells are part of the body wall fragment. In summary, the identification of genes with restricted expression patterns using whole genome profiling suggests that many genes with potential roles in wing disc development remain to be characterized.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (4)
	pan^ΔN.Scer\UAS Scer\GAL4^71B Scer\GAL4^bbg-C96 wg^Scer\UAS.cLa
Constructs (2)
	P{UAS-pan.dTCFΔN} P{UAS-wg.L}
Genes (150)
	Act57B ana Ance apt Arc1 B babos bi BM-40-SPARC bond C15 Cg25C CG1572 CG2663 CG3770 CG4386 CG5391 CG5397 CG5758 CG6234 CG7860 CG8483 CG9008 CG9192 CG9312 CG9338 CG9593 CG9747 CG9812 CG10126 CG10200 CG11370 CG11835 CG12481 CG13023 CG13044 CG13053 CG14301 CG15353 CG16820 CG16868 CG17278 CG17549 CG30069 CG31705 CG31999 CG33970 CG34380 CG42673 CG42749 Clect27 Cpr66D Cpr78E cv-2 Cyp310a1 Dll Doc1 Doc2 Drip drm dve E(spl)m6-BFM ec Ect3 ed Ef1α100E egr en eyg Fas2 Fbp1 Gasp gk Gld Grip Gs2 hh Him Hs6st htl Idgf4 if ImpL2 Inos inv kn kon Lcp1 LpR1 Lsd-2 Mal-A5 Mal-A6 mas Mef2 Mes2 mey nab Nep1 NetA Nox nub nvy Obp56a Obp56d Obp99a odd opa out pan pdm2 Phk-3 pip Plod pnr Poxn pr-set7 Pvf2 pyr regucalcin RhoGAP18B rn scb Scer\GAL4 sob Sox15 Sox102F SP1029 sr ss stumps tey ths toe Traf4 Tre1 trol Tsp tup TwdlE twi Ugt58Fa Ugt86Di vg vkg vvl wg wgn wun2 zfh1 zfh2
Insertions (2)
	P{GawB}71B P{GawB}bbg^C96