Reference Report

Reference
Citation	Ner, S.S., Harrington, M.J., Grigliatti, T.A. (2002). A role for the Drosophila SU(VAR)3-9 protein in chromatin organization at the histone gene cluster and in suppression of position-effect variegation. Genetics 162(4): 1763--1774. (Export to RIS)
FlyBase ID	FBrf0155808
Publication Type	Research paper
PubMed ID	12524347
PubMed Abstract	Mutations in the gene for Su(var)3-9 are dominant suppressors of position-effect variegation (PEV). We show that SU(VAR)3-9 is a chromatin-associated protein and identify the large multicopy histone gene cluster (HIS-C) as one of its target loci. The organization of nucleosomes over the entire HIS-C region is altered in Su(var)3-9 mutants and there is a concomitant increase in expression of the histone genes. SU(VAR)3-9 is a histone H3 methyltransferase and, using chromatin immunoprecipitation, we show that SU(VAR)3-9 is present at the HIS-C locus and that the histone H3 at the HIS-C locus is methylated. We propose that SU(VAR)3-9 is involved in packaging HIS-C into a distinct chromatin domain that has some of the characteristics of beta-heterochromatin. We suggest that methylation of histone H3 is important for the chromatin structure at HIS-C. The chromosomal deficiency for the HIS-C is also a suppressor of PEV. In contrast to what might be expected, we show that hemizygosity for the HIS-C locus leads to a substantial increase in the histone transcripts.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (3)
	Df(2L)DS5 Df(2L)DS6 In(1)w^m4
Alleles (8)
	Su(var)3-9³¹¹ Su(var)3-9³¹⁹ Su(var)3-9³³⁰ Su(var)3-9^P17 Su(var)3-9^P17-r2 Su(var)3-9^P17-r12 Su(var)3-9^P25 Su(var)3-9^P25-r2
Genes (6)
	His1 His2A His2B His3 His4 Su(var)3-9
Insertions (2)
	P{}Su(var)3-9^P17 P{}Su(var)3-9^P25