Open Close
Reference
Citation
Bilder, D., Schober, M., Perrimon, N. (2003). Integrated activity of PDZ protein complexes regulates epithelial polarity.  Nat. Cell Biol. 5(1): 53--58.
FlyBase ID
FBrf0155998
Publication Type
Research paper
Abstract

Polarized cells contain numerous membrane domains, but it is unclear how the formation of these domains is coordinated to create a single integrated cell architecture. Genetic screens of Drosophila melanogaster embryos have identified three complexes, each containing one of the PDZ domain proteins--Stardust (Sdt), Bazooka (Baz) and Scribble (Scrib)--that control epithelial polarity and formation of zonula adherens. We find that these complexes can be ordered into a single regulatory hierarchy that is initiated by cell adhesion-dependent recruitment of the Baz complex to the zonula adherens. The Scrib complex represses apical identity along basolateral surfaces by antagonizing Baz-initiated apical polarity. The Sdt-containing Crb complex is recruited apically by the Baz complex to counter antagonistic Scrib activity. Thus, a finely tuned balance between Scrib and Crb complex activity sets the limits of the apical and basolateral membrane domains and positions cell junctions. Our data suggest a model in which the maturation of epithelial cell polarity is driven by integration of the sequential activities of PDZ-based protein complexes.

PubMed ID
PubMed Central ID
Related Publication(s)
Note

A genetic hierarchy controlling cell polarity.
Johnson and Wodarz, 2003, Nat. Cell Biol. 5(1): 12--14 [FBrf0159239]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Cell Biol.
    Title
    Nature Cell Biology
    Publication Year
    1999-
    ISBN/ISSN
    1465-7392 1476-4679
    Data From Reference
    Aberrations (1)
    Alleles (10)
    Genes (15)
    Insertions (1)