Reference Report

Reference
Citation	Betschinger, K., Mechler, B., Knoblich, J.A. (2003). The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl. Nature 422(6929): 326--330. (Export to RIS)
FlyBase ID	FBrf0157155
Publication Type	Research paper
PubMed ID	12629552
PubMed Abstract	To generate different cell types, some cells can segregate protein determinants into one of their two daughter cells during mitosis. In Drosophila neuroblasts, the Par protein complex localizes apically and directs localization of the cell fate determinants Prospero and Numb and the adaptor proteins Miranda and Pon to the basal cell cortex, to ensure their segregation into the basal daughter cell. The Par protein complex has a conserved function in establishing cell polarity but how it directs proteins to the opposite side is unknown. We show here that a principal function of this complex is to phosphorylate the cytoskeletal protein Lethal (2) giant larvae (Lgl; also known as L(2)gl). Phosphorylation by Drosophila atypical protein kinase C (aPKC), a member of the Par protein complex, releases Lgl from its association with membranes and the actin cytoskeleton. Genetic and biochemical experiments show that Lgl phosphorylation prevents the localization of cell fate determinants to the apical cell cortex. Lgl promotes cortical localization of Miranda, and we propose that phosphorylation of Lgl by aPKC at the apical neuroblast cortex restricts Lgl activity and Miranda localization to the opposite, basal side of the cell.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (8)
	aPKC^dsRNA.cBa aPKC^Scer\UAS.cBa aPKC^ΔN.Scer\UAS l(2)gl^{3A.Scer\UAS.T:Hsap\MYC} l(2)gl^3A.Scer\UAS l(2)gl^Scer\UAS.cBa l(2)gl^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}
Constructs (9)
	P{matα4-GAL-VP16} P{UAS-aPKC.B} P{UAS-aPKC.Myc} P{UAS-aPKC.ΔN.Myc} P{UAS-aPKC.ΔN} P{UAS-l(2)gl.3A.Myc} P{UAS-l(2)gl.3A} P{UAS-l(2)gl.B} P{UAS-l(2)gl.Myc}
Genes (15)
	aPKC baz l(2)gl mira par-6 pon Scer\GAL4 Syx1A T:Hsap\MYC T:Hsim\VP16 zip αTub67C αTub84B αTub84D αTub85E