Reference Report

Reference
Citation	Somers, W.G., Saint, R. (2003). A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis. Dev. Cell 4(1): 29--39. (Export to RIS)
FlyBase ID	FBrf0158856
Publication Type	Research paper
PubMed ID	12530961
PubMed Abstract	The mechanism that positions the cytokinetic contractile ring is unknown, but derives from the spindle midzone. We show that an interaction between the Rho GTP exchange factor, Pebble, and the Rho family GTPase-activating protein, RacGAP50C, connects the contractile ring to cortical microtubules at the site of furrowing in D. melanogaster cells. Pebble regulates actomyosin organization, while RacGAP50C and its binding partner, the Pavarotti kinesin-like protein, regulate microtubule bundling. All three factors are required for cytokinesis. As furrowing begins, these proteins colocalize to a cortical equatorial ring. We propose that RacGAP50C-Pavarotti complexes travel on cortical microtubules to the cell equator, where they associate with the Pebble RhoGEF to position contractile ring formation and coordinate F-actin and microtubule remodeling during cytokinesis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Alleles (23)
	Cdc42⁴ Cdc42^dsRNA.cSa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Mtl^dsRNA.cSa Mtl^Δ pav^B200 pbl² pbl^dsRNA.cSa pbl^{Scer\UAS.T:Avic\GFP} pbl^{ΔDH497-549.Scer\UAS} Rac1^dsRNA.cSa Rac1^J11 Rac2^dsRNA.cSa Rac2^Δ Rho1^72O Rho1^dsRNA.cSa RhoL^dsRNA.cSa Scer\GAL4^en-e16E tum^dsRNA.cSa tum^{dsRNA.Scer\UAS.cSa} tum^{Scer\UAS.T:Hsap\MYC} tum^{ΔEIE.Scer\UAS.T:Hsap\MYC} tum^{ΔYRL.Scer\UAS.T:Hsap\MYC}
Constructs (7)
	P{UAS-mCD8::GFP.L} P{UAS-pbl::GFP} P{UAS-Pbl.ΔDH497-549} P{UAS-RacGAP50C.MYC} P{UAS-RacGAP50C.RNAi} P{UAS-RacGAP50C.ΔEIE.MYC} P{UAS-RacGAP50C.ΔYRL.MYC}
Genes (18)
	Cdc42 dia Mmus\Cd8a Mtl pav pbl pnut Rac1 Rac2 Rho1 RhoL Scer\GAL4 scra sqh T:Avic\GFP T:Hsap\MYC tum zip
Insertions (1)
	P{en2.4-GAL4}e16E