Reference Report

Reference
Citation	Yuasa, Y., Okabe, M., Yoshikawa, S., Tabuchi, K., Xiong, W.C., Hiromi, Y., Okano, H. (2003). Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors. Development 130(11): 2419--2428. (Export to RIS)
FlyBase ID	FBrf0158889
Publication Type	Research paper
PubMed ID	12702656
PubMed Abstract	In Drosophila, cell-fate determination of all neuroectoderm-derived glial cells depends on the transcription factor Glial cells missing (GCM), which serves as a binary switch between the neuronal and glial cell fates. Because the expression of GCM is restricted to the early phase of glial development, other factors must be responsible for the terminal differentiation of glial cells. Expression of three transcription factors, Reversed Polarity (REPO), Tramtrack p69 (TTK69) and PointedP1 (PNTP1), is induced by GCM in glial cells. REPO is a paired-like homeodomain protein, expressed exclusively in glial cells, and is required for the migration and differentiation of embryonic glial cells. To understand how REPO functions in glial terminal differentiation, we have analyzed the mechanism of gene regulation by REPO. We show that REPO can act as a transcriptional activator through the CAATTA motif in glial cells, and define three genes whose expression in vivo depends on REPO function. In different types of glial cells, REPO can act alone, or cooperate with either TTK69 or PNTP1 to regulate different target genes. Coordination of target gene expression by these three transcription factors may contribute to the diversity of glial cell types. In addition to promoting glial differentiation, we found that REPO is also necessary to suppress neuronal development, cooperating with TTK69. We propose that REPO plays a key role in both glial development and diversification.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (39)
	Ecol\lacZ^{ftz.2X21F.HDS} Ecol\lacZ^loco-rC56 Ecol\lacZ^M84 Ecol\lacZ^pnt-rM254 Ecol\lacZ^ttk-00219 gcm^Act5C.PY gcm^Scer\UAS.cHa pnt^P1.Scer\UAS pnt^P1.Y.Act5C pnt^Δ88 Ppyr\LUC^5x.Scer\UAS Ppyr\LUC^CAATTA Ppyr\LUC^CAGTTA Ppyr\LUC^hs.PYb Ppyr\LUC^loco.AEE* Ppyr\LUC^loco.AEE Ppyr\LUC^loco.AES repo^4e19 repo⁶⁴ repo^{114.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{219.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{303.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{524.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^Act5C.PY repo^{Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^Scer\UAS.cYa repo^{Δ33.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{Δ123.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{Δ197.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{Δ302.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^{Δ451.Act5C.T:Hsap\MYC,T:Scer\GAL4} repo^Δbox.Act5C Scer\GAL4^{Act5C.T:Hsap\MYC} Scer\GAL4^en-e16E Scer\GAL4^sca-T3 ttk^1e11 ttk^B330 ttk^p69.Scer\UAS w^+mC
Constructs (5)
	P{2X21F} P{UAS-gcm.H} P{UAS-pnt.P1} P{UAS-repo.Y} P{UAS-ttk.p69}
Genes (11)
	Ecol\lacZ elav gcm pnt Ppyr\LUC repo Scer\GAL4 T:Hsap\MYC T:Scer\GAL4 ttk w
Insertions (6)
	P{A92}pnt^rM254 P{en2.4-GAL4}e16E P{GawB}sca^T3 P{lacW}M84 P{lacZ-un1}loco^rC56 P{PZ}ttk⁰⁰²¹⁹