Reference Report

Reference
Citation	MacKay, J.O., Soanes, K.H., Srivastava, A., Simmonds, A., Brook, W.J., Bell, J.B., Bell, J.B. (2003). An in vivo analysis of the vestigial gene in Drosophila melanogaster defines the domains required for vg function. Genetics 163(4): 1365--1373. (Export to RIS)
FlyBase ID	FBrf0158979
Publication Type	Research paper
PubMed ID	12702681
PubMed Abstract	Considerable evidence indicates an obligate partnership of the Drosophila melanogaster Vestigial (VG) and Scalloped (SD) proteins within the context of wing development. These two proteins interact physically and a 56-amino-acid motif within VG is necessary and sufficient for this binding. While the importance of this SD-binding domain has been clearly demonstrated both in vitro and in vivo, the remaining portions of VG have not been examined for in vivo function. Herein, additional regions within VG were tested for possible in vivo functions. The results identify two additional domains that must be present for optimal VG function as measured by the loss of ability to rescue vg mutants, to induce ectopic sd expression, and to perform other normal VG functions when they are deleted. An in vivo study such as this one is fundamentally important because it identifies domains of VG that are necessary in the cellular context in which wing development actually occurs. The results also indicate that an additional large portion of VG, outside of these two domains and the SD-binding domain, is dispensable in the execution of these normal VG functions.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (25)
	Avic\GFP^Scer\UAS.cUa Ecol\lacZ^sd-ETX4 Scer\GAL4^Act5C.PI Scer\GAL4^ey.PH Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Act5C} Scer\GAL4^vg.PM vg¹ vg^83b27 vg^83b27-R vg^Scer\UAS.cKa vg^{Δ1-2.Scer\UAS} vg^{Δ1-4.Scer\UAS} vg^{Δ2-8.Scer\UAS} vg^{Δ2-10.Scer\UAS} vg^{Δ4-8.Scer\UAS} vg^{Δ4-10.Scer\UAS} vg^{Δ5'-5.Scer\UAS} vg^{Δ5'-6.Scer\UAS} vg^{Δ5'-7.Scer\UAS} vg^{Δ5'-9.Scer\UAS} vg^{Δ7-8.Scer\UAS} vg^{Δ8-9.Scer\UAS} vg^{Δ9-10.Scer\UAS} w^+mC
Constructs (19)
	P{Act5C-GAL4} P{AyGAL4} P{GAL4-ey.H} P{GAL4-vg.M} P{UAS-GFP.U} P{UAS-vg.K} P{UAS-vg.Δ1-2} P{UAS-vg.Δ1-4} P{UAS-vg.Δ2-8} P{UAS-vg.Δ2-10} P{UAS-vg.Δ4-8} P{UAS-vg.Δ4-10} P{UAS-vg.Δ5'-5} P{UAS-vg.Δ5'-6} P{UAS-vg.Δ5'-7} P{UAS-vg.Δ5'-9} P{UAS-vg.Δ7-8} P{UAS-vg.Δ8-9} P{UAS-vg.Δ9-10}
Genes (6)
	Avic\GFP Ecol\lacZ Scer\GAL4 vg w wg
Insertions (2)
	P{GawB}ptc^559.1 P{lArB}sd^ETX4