Reference Report

Reference
Citation	Wucherpfennig, T., Wilsch-Brauninger, M., Gonzalez-Gaitan, M. (2003). Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release. J. Cell Biol. 161(3): 609--624. (Export to RIS)
FlyBase ID	FBrf0159035
Publication Type	Research paper
PubMed ID	12743108
PubMed Abstract	During constitutive endocytosis, internalized membrane traffics through endosomal compartments. At synapses, endocytosis of vesicular membrane is temporally coupled to action potential-induced exocytosis of synaptic vesicles. Endocytosed membrane may immediately be reused for a new round of neurotransmitter release without trafficking through an endosomal compartment. Using GFP-tagged endosomal markers, we monitored an endosomal compartment in Drosophila neuromuscular synapses. We showed that in conditions in which the synaptic vesicles pool is depleted, the endosome is also drastically reduced and only recovers from membrane derived by dynamin-mediated endocytosis. This suggests that membrane exchange takes place between the vesicle pool and the synaptic endosome. We demonstrate that the small GTPase Rab5 is required for endosome integrity in the presynaptic terminal. Impaired Rab5 function affects endo- and exocytosis rates and decreases the evoked neurotransmitter release probability. Conversely, Rab5 overexpression increases the release efficacy. Therefore, the Rab5-dependent trafficking pathway plays an important role for synaptic performance.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(2L)Rab5²
Alleles (19)
	Avic\GFP^{Scer\UAS.T:Hsap\MYC,T:Mmus\2xFYVE} Axud1² Mmus\Hgs^{2xFYVE.Scer\UAS.T:Hsap\MYC} Rab5² Rab5³ Rab5⁴ Rab5⁰⁰²³¹ Rab5^+tWa Rab5^k08232 Rab5^{S43N.Scer\UAS.T:Avic\GFP} Rab5^{S43N.Scer\UAS} Rab5^Scer\UAS.cEa Rab5^{Scer\UAS.T:Avic\GFP-EGFP} Rab5^unspecified Scer\GAL4^elav-C155 Scer\GAL4^en-e16E shi¹ T:Avic\GFP^EGFP w^+mC
Constructs (5)
	P{Rab5^+tWa} P{UAS-GFP-myc-2xFYVE} P{UAS-GFP-Rab5} P{UAS-GFP-Rab5S43N} P{UAS-myc-2xFYVE}
Genes (15)
	AP-2α Avic\GFP Axud1 brp Csp eys Fas2 Mmus\Hgs Rab5 Scer\GAL4 shi T:Avic\GFP T:Hsap\MYC T:Mmus\2xFYVE w
Insertions (1)
	P{PZ}Rab5⁰⁰²³¹