Reference Report

Reference
Citation	Suster, M.L., Martin, J.R., Sung, C., Robinow, S. (2003). Targeted expression of tetanus toxin reveals sets of neurons involved in larval locomotion in Drosophila. J. Neurobiol. 55(2): 233--246. (Export to RIS)
FlyBase ID	FBrf0159050
Publication Type	Research paper
PubMed ID	12672020
PubMed Abstract	The Drosophila larva is widely used for studies of neuronal development and function, yet little is known about the neuronal basis of locomotion in this model organism. Drosophila larvae crawl over a plain substrate by performing repetitive waves of forward peristalsis alternated by brief episodes of head swinging and turning. To identify sets of central and peripheral neurons required for the spatial or temporal pattern of larval locomotion, we blocked neurotransmitter release from defined populations of neurons by targeted expression of tetanus toxin light chain (TeTxLC) with the GAL4/UAS system. One hundred fifty GAL4 lines were crossed to a UAS-TeTxLC strain and a motion-analysis system was used to identify larvae with abnormal movement patterns. Five lines were selected that show discrete locomotor defects (i.e., increased turning and pausing) and these defects are correlated with diverse sets of central neurons. One line, 4C-GAL4, caused an unusual circling behavior that is correlated with approximately 200 neurons, including dopaminergic and peptidergic interneurons. Expression of TeTxLC in all dopaminergic and serotonergic but not in peptidergic neurons, caused turning deficits that are similar to those of 4C-GAL4/TeTxLC larvae. The results presented here provide a basis for future genetic studies of motor control in the Drosophila larva.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurobiol.
Title	Journal of Neurobiology
Publication Year	1969-
ISBN/ISSN	0022-3034
Data from Reference
Alleles (31)
	Avic\GFP^{Scer\UAS.T:Ecol\lacZ,T:SV40\nls2} Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} Ctet\TeTxLC^TNT.Scer\UAS Ecol\lacZ^Scer\UAS.cUa Ecol\lacZ^{Scer\UAS.O.T:Btau\MAPT} Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} mbm^N337 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Phm^k07623 Scer\GAL4^4C Scer\GAL4³²⁶ Scer\GAL4³⁴³ Scer\GAL4^acj6-PG63 Scer\GAL4^c401 Scer\GAL4^c819 Scer\GAL4^Ddc.PL Scer\GAL4^dimm-929 Scer\GAL4^eve.RRK Scer\GAL4^Fmrf.PS Scer\GAL4^H24 Scer\GAL4^how-24B Scer\GAL4^Jan115 Scer\GAL4^Jan204 Scer\GAL4^lilli-189Y Scer\GAL4^Mz604 Scer\GAL4^Mz689 Scer\GAL4^Mz840 Scer\GAL4^Mz972-21 Scer\GAL4^OK72 Scer\GAL4^OK83 Scer\GAL4^Tab2-201Y
Constructs (11)
	P{Ddc-GAL4.L} P{eve-GAL4.RRK} P{Fmrf-GAL4.S} P{GAL4} P{UAS-GFP::lacZ.nls} P{UAS-Hsap\KCNJ2.EGFP} P{UAS-lacZ.btau.YES} P{UAS-lacZ.U} P{UAS-mCD8::GFP.L} P{UAS-TeTxLC.(-)V} P{UAS-TeTxLC.tnt}
Genes (12)
	acj6 Avic\GFP Ctet\TeTxLC Ecol\lacZ how Hsap\KCNJ2 mbm Mmus\Cd8a Phm ple Scer\GAL4 Tab2
Insertions (20)
	P{Fmrf-GAL4.S}FG5 P{GAL4}4C P{GAL4}326 P{GAL4}343 P{GAL4}H24 P{GAL4}Mz604 P{GAL4}Mz689 P{GAL4}Mz972-21 P{GAL4}OK83 P{GawB}acj6^PG63 P{GawB}c401 P{GawB}c819 P{GawB}dimm⁹²⁹ P{GawB}how^24B P{GawB}Jan115 P{GawB}Jan204 P{GawB}lilli^189Y P{GawB}Mz840 P{GawB}OK72 P{GawB}Tab2^201Y
Transcripts (9)
	Scer\GAL4^4CRA Scer\GAL4^c819RA Scer\GAL4^H24RA Scer\GAL4^how-24BRA Scer\GAL4^Jan115RA Scer\GAL4^Jan204RA Scer\GAL4^Mz689RA Scer\GAL4^Mz972-21RA Scer\GAL4^Tab2-201YRA