Reference Report

Reference
Citation	Lee, G., Hall, J.C., Park, J.H. (2002). Doublesex gene expression in the central nervous system of Drosophila melanogaster. J. Neurogenet. 16(4): 229--248. (Export to RIS)
FlyBase ID	FBrf0159058
Publication Type	Research paper
PubMed ID	12745633
PubMed Abstract	Despite several behavior-genetic studies that have suggested roles played by doublesex (dsx) in neural tissues, it has not been demonstrated that the products of this gene are actually present in the central nervous system (CNS). In this report, we describe the cellular, spatial, and temporal expression patterns of dsx gene products in the developing and adult CNS by applying RT-PCR and immunohistochemical procedures. dsx gene products were detected in the CNS of 3rd-instar larvae, pupae, and adults. DSX-immunoreactive signals were observed within the brain and in both the thoracic plus abdominal ganglia of the ventral nerve cord. Most, but not all, cells inferred to contain DSX proteins (by the results of genetic controls for antibody specificity) were further determined to be neurons (by coexpression of a protein that marks such CNS cell types). Temporally varying expression of DSX was most prominently observed in the rapidly metamorphosing early and mid-pupal stages, suggesting that this gene contributes to establishment of sexually dimorphic neuronal structures which subserve adult sexual behaviors. Elements of the spatial and temporal patterns of DSX immunoreactivity also imply that sexually dimorphic dsx expression in certain neuronal clusters within the adult CNS could participate in ongoing operations of the mature nervous system with respect to the courtship behaviors that are affected by dsx mutations.
DOI
Related Publication(s)
Personal communication to FlyBase	P{UAS-dsx.M} and P{UAS-dsx.F}. Park and Burtis, 2013.3.21, P{UAS-dsx.M} and P{UAS-dsx.F}. [FBrf0221249]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Neurogenet.
Title	Journal of Neurogenetics
Publication Year	1983-
ISBN/ISSN	0167-7063
Data from Reference
Aberrations (2)
	Df(3R)dsx15 In(3R)dsx²³
Alleles (7)
	Avic\GFP^{S65T.Scer\UAS} dsx^{Scer\UAS.female} dsx^{Scer\UAS.male} Ecol\lacZ^Yp1.4o Scer\GAL4^P2.4.Pdf Scer\GAL4^r4 tra¹
Constructs (6)
	P{Pdf-GAL4.P2.4} P{r4-GAL4} P{UAS-dsx.F} P{UAS-dsx.M} P{UAS-GFP.S65T} P{Yp1-lacZ.o4}
Genes (7)
	Avic\GFP dsx Ecol\lacZ elav Pdf Scer\GAL4 tra
Natural transposons (1)
	P-element