Reference Report

Reference
Citation	Kai, T., Spradling, A. (2003). An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells. Proc. Natl. Acad. Sci. U.S.A. 100(8): 4633--4638. (Export to RIS)
FlyBase ID	FBrf0159297
Publication Type	Research paper
PubMed ID	12676994
PubMed Abstract	Stem cells are thought to reside in regulatory microenvironments ("niches") generated by stable stromal neighbors. To investigate the significance of empty niches vacated by stem cell loss, we studied Drosophila ovarioles, which maintain two to three germ-line stem cells in a niche requiring adhesive stromal cap cells and Decapentaplegic signals. After experimentally emptying the germ-line stem cell niche, cap cell activity persists for several weeks. Initially, somatic inner germarium sheath cells enter the empty niche, respond to Dpp, but fail to divide. Subsequently, follicle cell progenitors, including somatic stem cells enter the niche, respond to Dpp, and proliferate as long as cap cells remain. Proliferation requires the normal hedgehog signal of the somatic stem cells as well as proximity to the niche. Thus, empty niches can persist, signal incoming cells, and support ectopic proliferation. Similar events may underlie some disease states.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (14)
	bam^hs.PO bam^Δ86 Dad^PZ dpp^hr4 dpp^hr56 dpp^Scer\UAS.cNa Ecol\lacZ⁰⁰⁷⁸ Ecol\lacZ¹⁴⁴⁴ Ecol\lacZ²⁹⁵⁴ Ecol\lacZ¹⁰⁶¹³ Ecol\lacZ^Dad-PZ Ecol\lacZ^hh-P30 hh^hs.PI hh^ts2
Constructs (3)
	P{hs-bam.O} P{HShh.I} P{UAS(-FRT)dpp.N}
Genes (8)
	bam Dad dpp Ecol\lacZ hh Mad ptc Scer\GAL4
Insertions (8)
	P{hs-bam.O}11d P{hs-bam.O}18d P{PZ}0078 P{PZ}1444 P{PZ}2954 P{PZ}10613 P{PZ}Dad^PZ P{PZ}hh^P30