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Citation	Correia, T., Papayannopoulos, V., Panin, V., Woronoff, P., Jiang, J., Vogt, T.F., Irvine, K.D. (2003). Molecular genetic analysis of the glycosyltransferase Fringe in Drosophila. Proc. Natl. Acad. Sci. USA 100(11): 6404--6409.
FlyBase ID	FBrf0159701
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	12743367
PubMed Abstract	Fringe proteins are beta1,3-N-acetylglucosaminyltransferases that modulate signaling through Notch receptors by modifying O-linked fucose on epidermal growth factor domains. Fringe is highly conserved, and comparison among 18 different Fringe proteins from 11 different species identifies a core set of 84 amino acids that are identical among all Fringes. Fringe is only distantly related to other glycosyltransferases, but analysis of the predicted Drosophila proteome identifies a set of four sequence motifs shared among Fringe and other putative beta1,3-glycosyltransferases. To gain functional insight into these conserved sequences, we genetically and molecularly characterized 14 point mutations in Drosophila fringe. Most nonsense mutations act as recessive antimorphs, raising the possibility that Fringe may function as a dimer. Missense mutations identify two distinct motifs that are conserved among beta1,3-glycosyltransferases, and that can be modeled onto key motifs in the crystallographic structures of bovine beta1,4-galactosyltransferase 1 and human glucuronyltransferase I. Other missense mutations map to amino acids that are conserved among Fringe proteins, but not among other glycosyltransferases, and thus may identify structural motifs that are required for unique aspects of Fringe activity.
Biosis	2003.313294
Zoological record	14001001381
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Language of publication	English
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Abbreviation	Proc. Natl. Acad. Sci. USA
Title	Proceedings of the National Academy of Sciences of the United States of America
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Volume range	1-
Year range	1915-
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Place of publication	Washington, DC
Language of publication	English
ISBN/ISSN	0027-8424
CODEN	PNASA6
Data from Reference
Aberrations (1)
	Df(3L)ri-XT1
Alleles (20)
	Avic\GFP^{S65T.Scer\UAS} Scer\GAL4^Kr.PM fng¹¹ fng¹²⁹ fng¹³ fng¹⁴ fng¹ fng² fng^35UZ-1 fng³ fng⁵² fng⁵ fng⁷ fng⁸⁰ fng⁸ fng^L19 fng^L73 fng^L81 fng^L83 fng^M69
Constructs (2)
	P{GAL4-Kr.C} P{UAS-GFP.S65T}
Genes (3)
	Avic\GFP Scer\GAL4 fng
Insertions (1)
	P{35UZ}fng^35UZ-1