Reference Report

Reference
Citation	Fuse, N., Hisata, K., Katzen, A.L., Matsuzaki, F. (2003). Heterotrimeric g proteins regulate daughter cell size asymmetry in Drosophila neuroblast divisions. Curr. Biol. 13(11): 947--954. (Export to RIS)
FlyBase ID	FBrf0159715
Publication Type	Research paper
External Crossreferences
PubMed ID	12781133
PubMed Abstract	Cell division often generates unequally sized daughter cells by off-center cleavages, which are due to either displacement of mitotic spindles or their asymmetry. Drosophila neuroblasts predominantly use the latter mechanism to divide into a large apical neuroblast and a small basal ganglion mother cell (GMC), where the neural fate determinants segregate. Apically localized components regulate both the spindle asymmetry and the localization of the determinants. Here, we show that asymmetric spindle formation depends on signaling mediated by the G beta subunit of heterotrimeric G proteins. G beta 13F distributes throughout the neuroblast cortex. Its lack induces a large symmetric spindle and causes division into nearly equal-sized cells with normal segregation of the determinants. In contrast, elevated G beta 13F activity generates a small spindle, suggesting that this factor suppresses spindle development. Depletion of the apical components also results in the formation of a small symmetric spindle at metaphase. Therefore, the apical components and G beta 13F affect the mitotic spindle shape oppositely. We propose that differential activation of G beta signaling biases spindle development within neuroblasts and thereby causes asymmetric spindles. Furthermore, the multiple equal cleavages of G beta mutant neuroblasts accompany neural defects; this finding suggests indispensable roles of eccentric division in assuring the stem cell properties of neuroblasts.
BIOSIS ID	2003.384254
Zoological Record ID
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Editors
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (16)
	Avic\GFP^{Scer\UAS.T:Ecol\lacZ,T:SV40\nls2} baz^dsRNA.cFa cta⁵ fog⁴ G-iα65A^{Q205L.Scer\UAS} G-iα65A^Scer\UAS.cFa Gβ13F::mira^Scer\UAS.cFa Gβ13F^f261 Gβ13F^Scer\UAS.cFa Gβ13F^Δ1-96A Gβ13F^Δ15 Gγ1^Scer\UAS.cFa raps^P89 Scer\GAL4^Act5C.PHb Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^pros.PMG
Constructs (9)
	P{Act5C-GAL4.Hb} P{GAL4-pros.MG} P{matα4-GAL-VP16} P{UAS-GFP::lacZ.nls} P{UAS-G-iα65A.F} P{UAS-G-iα65A.Q205L} P{UAS-Gβ13F.?F} P{UAS-Gγ1.F} P{UAS-MirN-Gβ13F}
Genes (27)
	ase Avic\GFP baz cas cta dpn eve fog G-iα65A Gβ13F Gβ13F::mira Gγ1 His3 mira Nrt pros raps Scer\GAL4 T:Ecol\lacZ T:Hsim\VP16 T:SV40\nls2 αTub67C αTub84B αTub84D αTub85E γTub23C γTub37C
Insertions (1)
	P{lacW}l(1)G0369^G0369