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Giniger, E. (2003.7.28). UAS-Dl, nrtM54, trio123.4 and sim-GAL4. 
FlyBase ID
FBrf0159722
Publication Type
Personal communication to FlyBase
Abstract
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Text of Personal Communication
Date: Mon, 28 Jul 2003  09:50:32  \-0700
To: Chihiro Yamada <cy200@XXXX>
From: Ed Giniger <eginiger@XXXX>
Subject: Re: FlyBase Query (cy1992)
>Dear Dr Giniger,
>
>I am currently curating your paper for FlyBase:
>
>Crowner et al. 2003, Curr. Biol. 13(11): 967--972
>
>I have a few questions I was hoping you could answer for me:
>
>UAS-Dl
>======
the UAS-Dl is the one used in the Doherty paper (what the paper
neglects to mention is that I'm the one who made it and gave it to
them)
>You use a UAS-Dl construct. We have 4 of these constructs in our
>records \- is it one of these?
>
>DlScer\UAS.cDa
> Doherty et al., 1996, Genes Dev. 10(4): 421--434
> Okajima and Irvine, 2002, Cell 111(6): 893--904
> Panin et al., 1997, Nature 387(6636): 908--912
>
>DlScer\UAS.cHa
> Jonsson and Knust, 1996, Dev. Genes Evol. 206(2): 91--101
> Klein et al., 1997, Dev. Biol. 189(1): 123--134
> Fanto and Mlodzik, 1999, Nature 397(6719): 523--526
>
>DlScer\UAS.cJa
> Baonza and Freeman, 2001, Development 128(20): 3889--3898
> Hukriede et al., 1997, Development 124(17): 3427--3437
> Lear et al., 1999, Mech. Dev. 88(2): 207--219
>
>DlScer\UAS.cLa
> Lee et al., 2000, Curr. Biol. 10(15): 931--934
> Li and Baker, 2001, Curr. Biol. 11(5): 330--338
>
>If you are not sure, can you give me some more information on the one
>that you used to help me identify it? Where did you get the line from?
>Has it been published before? If so where?
>
>nrtM54
>========
the source of nrtM54 is discussed in the text. The mutant that has
traditionally been called dabM54 (from Gertler and Hoffmann) is
actually a mutation in nrt, as was shown recently by Liebl et al
(Development, 2003; also referenced in the text)
>We do not have a nrt allele with this name. Can you give me some more
>information on this allele? Where did you get the line from? Has it
>been published before? If so where and under what name? If its a new
>allele can you give me some details? What was the mutagen used to make
>this allele? What are the molecular details of this allele?
>
>trio123.4
>===========
trio 123.4 is from Eric Liebl. What he told me is that it is an
imprecise excision of the P-element in l(3)036810
>We do not have a trio allele with this name. Can you give me some more
>information on this allele? Where did you get the line from? Has it
>been published before? If so where and under what name? If its a new
>allele can you give mesome details? What was the mutagen used to make
>this allele? What are the molecular details of this allele?
>
>sim-GAL4
>========
>You use a sim-GAL4 line in your paper. We have 2 of these constructs in our
>records \- is it one of these?
We got sim-GAL4 from Steve Crews, so my guess would be that it is the
P3.7, but that is just a guess.
>
>Scer\GAL4sim.P3.7
> Battye et al., 1999, Development 126(11): 2475--2481
> Georgiou and Tear, 2002, Development 129(12): 2947--2956
> Kidd et al., 1998, Neuron 20(1): 25--33
> Kramer et al., 2001, Science 292(5517): 737--740
> Lanoue and Jacobs, 1999, Dev. Genet. 25(4): 321--330
> Noordermeer et al., 1998, Neuron 21(5): 991--1001
> Sedaghat et al., 2002, Development 129(11): 2591--2606
> Xiao et al., 1996, Mech. Dev. 58(1-2): 65--74
> Scer\GAL4 gene expression is driven by 3.7kb BamHI fragment of
> sim 5' flanking DNA (and 18 amino acids of coding sequence)
> containing the early sim promoter and 5' flanking sequences.
>
>Scer\GAL4sim.PS
> Becker et al., 1997, Development 124(13): 2615--2622
> Bossing and Brand, 2002, Development 129(18): 4205--4218
> Dorfman et al., 2002, Dev. Biol. 252(1): 119--126
> Golembo et al., 1996, Development 122(11): 3363--3370
> Hummel et al., 2000, Neuron 26(2): 357--370
> Luer et al., 1997, Development 124(14): 2681--2690
> Sanchez Soriano and Russell, 1998, Development 125(20): 3989--3996
> Scholz et al., 1997, Mech. Dev. 62(1): 79--91
> Vorbruggen and Jackle, 1997, Proc. Natl. Acad. Sci. USA
>94(16): 8606--8611
> 3.5kb EcoRV sim promoter fragment drives expression of
> Scer\GAL4.
>
>If you are not sure, can you give me some more information on the one
>that you used to help me identify it? Where did you get the line from?
>Has it been published before? If so where?
>
>Any new information you give me will be curated as a personal
>communication from you to FlyBase, if thats fine with you.
Feel free to quote me as a personal communication for any of the
information above.
>Best wishes,
>
>Chihiro
>
>----------------------------------------------------------------------
>Chihiro Yamada.
>
>FlyBase (Cambridge), http://fbserver.gen.cam.ac. uk:7081 /
>Department of Genetics,
>University of Cambridge, email: c.yamadagen.cam.ac.uk
>Downing Street, Tel : 01223-333963
>Cambridge, CB2 3EH, FAX : 01223-333992
>United Kingdom. Memes don't exist. Spread the Word.
>----------------------------------------------------------------------
DOI
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