Reference Report

Reference
Citation	Bastock, R., Strutt, H., Strutt, D. (2003). Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. Development 130(13): 3007--3014. (Export to RIS)
FlyBase ID	FBrf0160398
Publication Type	Research paper
PubMed ID	12756182
PubMed Abstract	Planar polarity decisions in the wing of Drosophila involve the assembly of asymmetric protein complexes containing the conserved receptor Frizzled. In this study, we analyse the role of the Van Gogh/strabismus gene in the formation of these complexes and cell polarisation. We find that the Strabismus protein becomes asymmetrically localised to the proximal edge of cells. In the absence of strabismus activity, the planar polarity proteins Dishevelled and Prickle are mislocalised in the cell. We show that Strabismus binds directly to Dishevelled and Prickle and is able to recruit them to membranes. Furthermore, we demonstrate that the putative PDZ-binding motif at the C terminus of Strabismus is not required for its function. We propose a two-step model for assembly of Frizzledcontaining asymmetric protein complexes at cell boundaries. First, Strabismus acts together with Frizzled and the atypical cadherin Flamingo to mediate apicolateral recruitment of planar polarity proteins including Dishevelled and Prickle. In the second phase, Dishevelled and Prickle are required for these proteins to become asymmetrically distributed on the proximodistal axis.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(2R)w45-30n
Alleles (21)
	dsh³ dsh^Scer\UAS.cNa Ecol\lacZ^Scer\UAS.cUa fz¹⁵ fz²³ fz²⁵ fz^{arm.T:Avic\GFP-EGFP} fz^Scer\UAS.cAa pk^pk-sple-13 pk^Scer\UAS.cGa Scer\GAL4^Act5C.PI Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Act5C} stan^E59 stan^Scer\UAS.cUa T:Avic\GFP^EGFP T:Avic\GFP^YFP Vang^{Act5C.T:Avic\GFP-YFP} Vang^Scer\UAS.cBa Vang^unspecified Vang^{ΔPBM.Act5C.T:Avic\GFP-YFP}
Constructs (11)
	P{Act5C-GAL4} P{Act5C-Vang.YFP} P{Act5C-Vang.ΔPBM.YFP} P{arm-fz.GFP} P{AyGAL4} P{UAS-dsh.N} P{UAS-fmi.U} P{UAS-fz.A} P{UAS-lacZ.U} P{UAS-pk.G} P{UAS-Stbm.B}
Genes (9)
	dlg1 dsh Ecol\lacZ fz pk Scer\GAL4 stan T:Avic\GFP Vang
Insertions (1)
	P{GawB}ptc^559.1