Reference Report

Reference
Citation	Dai, P., Akimaru, H., Ishii, S. (2003). A hedgehog-responsive region in the Drosophila wing disc is defined by debra-mediated ubiquitination and lysosomal degradation of Ci. Dev. Cell 4(6): 917--928. (Export to RIS)
FlyBase ID	FBrf0160468
Publication Type	Research paper
PubMed ID	12791275
PubMed Abstract	Transcription factor Ci mediates Hedgehog (Hh) signaling to determine the anterior/posterior (A/P) compartment of Drosophila wing disc. While Hh-inducible genes are expressed in A compartment cells abutting the A/P border, it is unclear how the boundaries of this region are established. Here, we have identified a Ci binding protein, Debra, that is expressed at relatively high levels in the band abutting the border of the Hh-responsive A compartment region. Debra mediates the polyubiquitination of full-length Ci, which then leads to its lysosomal degradation. Debra is localized in the multivesicular body, suggesting that the polyubiquitination of Ci directs its sorting into lysosome. Thus, Debra defines the border of the Hh-responsive region in the A compartment by inducing the lysosomal degradation of Ci.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (1)
	Df(2L)PM44
Alleles (22)
	Avic\GFP^{S65T.Scer\UAS} ci^76.Scer\UAS ci^{m1-4.Scer\UAS.T:Ivir\HA1} ci^Scer\UAS.cAa ci^{Scer\UAS.T:Ivir\HA1} ci^{U.Scer\UAS.T:Ivir\HA1} dbr^EP9 dbr^EP0456 Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^dpp-P1552 Ecol\lacZ^hh-P30 Ecol\lacZ^ptc-H84 Mmus\Pkaca^mC.Scer\UAS Scer\GAL4^71B Scer\GAL4^Act5C.PI Scer\GAL4^C-765 Scer\GAL4^hs.PB Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Act5C} slmb¹ slmb² slmb^dsRNA.cPa
Constructs (11)
	P{Act5C-GAL4} P{AyGAL4} P{BS3.0} P{GAL4-Hsp70.PB} P{UAS-ci.76} P{UAS-ci.A} P{UAS-ci.HA.m1-4} P{UAS-ci.HA} P{UAS-ci.U.HA} P{UAS-GFP.S65T} P{UAS-PKA.mC}
Genes (12)
	Avic\GFP ci dbr dpp Ecol\lacZ en hh Mmus\Pkaca ptc Scer\GAL4 slmb T:Ivir\HA1
Insertions (8)
	P{A92}ptc^H84 P{EP}dbr^EP9 P{EP}dbr^EP0456 P{GawB}71B P{GawB}C-765 P{GawB}ptc^559.1 P{PZ}hh^P30 P{PZ}P1552