Reference Report

Reference
Citation	Erhardt, S., Lyko, F., Ainscough, J.F., Surani, M.A., Paro, R. (2003). Polycomb-group proteins are involved in silencing processes caused by a transgenic element from the murine imprinted H19/Igf2 region in Drosophila. Dev. Genes Evol. 213(7): 336--344. (Export to RIS)
FlyBase ID	FBrf0160509
Publication Type	Research paper
PubMed ID	12750886
PubMed Abstract	A subset of autosomal genes undergo genomic imprinting which results in expression from only the paternal or maternal chromosome. While this phenomenon is restricted to mammals and angiosperms, the underlying silencing mechanisms appear to be evolutionarily conserved. A biallelically unmethylated DNaseI hypersensitive region (A6-A4) between the imprinted Igf2 and H19 genes is conserved in humans and mice and functions as a tissue-specific maintenance element for the imprinted growth factor IGF2. In order to analyse A6-A4 for potentially conserved transcriptional maintenance properties, we have generated transgenic Drosophila harbouring the element in a reporter construct. These flies depicted silencing of the reporter genes lacZ and mini -white. The silenced state of the mini -white gene showed variegation and sensitivity to temperature changes. In addition, two members of the conserved Polycomb group, Enhancer of zeste and Posterior sex combs, were needed for repression. Polycomb group proteins are essential for gene silencing during development. Our results indicate that Polycomb group proteins may also be involved in the regulation of mammalian imprinted genes.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Genes Evol.
Title	Development genes and evolution
Publication Year	1996-
ISBN/ISSN	0949-944X
Data from Reference
Aberrations (1)
	Df(2R)vg-B
Alleles (25)
	brm² E(z)¹ E(z)⁵ E(z)^ts Ecol\lacZ^{Mmus\H19.UZA.Scer\UAS} Ecol\lacZ^UZC.Scer\UAS JIL-1⁶ Pc¹⁵ Pp1-87B¹ Psc^1.d19 Psc^1.d20 Psc¹ Scer\GAL4^1032.hx Su(var)2-1¹ Su(var)3-3¹⁶ Su(var)3-9⁵ Su(var)3-9⁷ Su(var)205⁵ Su(z)2⁵ Su(z)2¹⁰¹ Trl^13C Trl⁶² trx^129.1 w^+* w^H19.UZA
Constructs (2)
	P{H19-lacZ.UZA} P{UZC.lacZ}
Genes (21)
	brm E(z) Ecol\lacZ JIL-1 mod(mdg4) Pc ph-d ph-p Pp1-87B Psc Sam-S Scer\GAL4 Su(var)2-1 Su(var)3-3 Su(var)3-7 Su(var)3-9 Su(var)205 Su(z)2 Trl trx w