Reference Report

Reference
Citation	Kelley, R.L., Kuroda, M.I. (2003). The Drosophila roX1 RNA gene can overcome silent chromatin by recruiting the male-specific lethal dosage compensation complex. Genetics 164(2): 565--574. (Export to RIS)
FlyBase ID	FBrf0160666
Publication Type	Research paper
PubMed ID	12807777
PubMed Abstract	The Drosophila MSL complex consists of at least six proteins and two noncoding roX RNAs that mediate dosage compensation. It acts to remodel the male's X chromatin by covalently modifying the amino terminal tails of histones. The roX1 and roX2 genes are thought to be nucleation sites for assembly and spreading of MSL complexes into surrounding chromatin where they roughly double the rates of transcription. We generated many transgenic stocks in which the roX1 gene was moved from its normal location on the X to new autosomal sites. Approximately 10% of such lines displayed unusual sexually dimorphic expression patterns of the transgene's mini-white eye-color marker. Males often displayed striking mosaic pigmentation patterns similar to those seen in position-effect variegation and yet most inserts were in euchromatic locations. In many of these stocks, female mini-white expression was very low or absent. The male-specific activation of mini-white depended upon the MSL complex. We propose that these transgenes are inserted in several different types of repressive chromatin environments that inhibit mini-white expression. Males are able to overcome this silencing through the action of the MSL complex spreading from the roX1 gene and remodeling the local chromatin to allow transcription. The potency with which an ectopic MSL complex overcomes silent chromatin suggests that its normal action on the X must be under strict regulation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	Df(3R)red-P93
Alleles (12)
	His3^39DE msl-1^Hsp83.PC msl-2^ey.hs Pc³ roX1^t4.9 Su(var)3-7^unspecified Su(var)205^unspecified Su(z)12^unspecified w^69C w^+mC w^B1-12 y^+mDint2
Constructs (3)
	P{GMroX1} P{Hsp83-msl-1.C} P{y⁺YEM2}
Genes (15)
	ara BicC E2f His3 msl-1 msl-2 Pc roX1 scrt Su(var)3-7 Su(var)205 Su(z)12 w y zfh1
Insertions (20)
	P{GMroX1}21C P{GMroX1}35E P{GMroX1}64A.2 P{GMroX1}75C P{GMroX1}77A P{GMroX1}80C P{GMroX1}84E P{GMroX1}93E P{GMroX1}95E P{GMroX1}98B P{GMroX1}99C P{GMroX1}99F P{GMroX1}102C P{GMroX1}ara^69C P{GMroX1}His3^39DE P{GMroX1}l(2)56Da^GMroX1 P{GMroX1}l(3)64Ao^GMroX1 P{GMroX1}l(3)82Cb^GMroX1 P{lacW}mirr^B1-12 P{y⁺YEM2}9