Reference Report

Reference
Citation	Sigrist, S.J., Reiff, D.F., Thiel, P.R., Steinert, J.R., Schuster, C.M. (2003). Experience-dependent strengthening of Drosophila neuromuscular junctions. J. Neurosci. 23(16): 6546--6556. (Export to RIS)
FlyBase ID	FBrf0160953
Publication Type	Research paper
PubMed ID	12878696
PubMed Abstract	The genetic analysis of larval neuromuscular junctions (NMJs) of Drosophila has provided detailed insights into molecular mechanisms that control the morphological and physiological development of these glutamatergic synapses. However, because of the chronic defects caused by mutations, a time-resolved analysis of these mechanisms and their functional relationships has been difficult so far. In this study we provide a first temporal map of some of the molecular and cellular key processes, which are triggered in wild-type animals by natural larval locomotor activity and then mediate experience-dependent strengthening of larval NMJs. Larval locomotor activity was increased either by chronically rearing a larval culture at 29 degrees C instead of 18 or 25 degrees C or by acutely transferring larvae from a culture vial onto agar plates. Within 2 hr of enhanced locomotor activity, NMJs showed a significant potentiation of signal transmission that was rapidly reversed by an induced paralysis of the temperature-sensitive mutant parats1. Enhanced locomotor activity was also associated with a significant increase in the number of large subsynaptic translation aggregates. After 4 hr, postsynaptic DGluR-IIA glutamate receptor subunits started to transiently accumulate in ring-shaped areas around synapses, and they condensed later on, after chronic locomotor stimulation at 29 degrees C, into typical postsynaptic patches. These NMJs showed a reduced perisynaptic expression of the cell adhesion molecule Fasciclin II, an increased number of junctional boutons, and significantly more active zones. Such temporal mapping of experience-dependent adaptations at developing wild-type and mutant NMJs will provide detailed insights into the dynamic control of glutamatergic signal transmission.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (1)
	Df(2L)cl-h4
Alleles (7)
	eag¹ GluRIIA^AD9 GluRIIA^Scer\UAS.cPa pAbp^k10109 para^ts1 Scer\GAL4^Mhc.PW Sh⁷
Constructs (2)
	P{GAL4-Mhc.W} P{UAS-GluRIIA.P}
Genes (8)
	eag eIF-4E Fas2 GluRIIA pAbp para Scer\GAL4 Sh
Insertions (1)
	P{lacW}pAbp^k10109