Reference Report

Reference
Citation	Subramanian, A., Prokop, A., Yamamoto, M., Sugimura, K., Uemura, T., Betschinger, J., Knoblich, J.A., Volk, T. (2003). Shortstop recruits EB1/APC1 and promotes microtubule assembly at the muscle-tendon junction. Curr. Biol. 13(13): 1086--1095. (Export to RIS)
FlyBase ID	FBrf0160988
Publication Type	Research paper
PubMed ID	12842007
PubMed Abstract	Shot (previously named Kakapo), is a Drosophila Plakin family member containing both Actin binding and microtubule binding domains. In Drosophila, it is required for a wide range of processes, including axon extension, dendrite formation, axonal terminal arborization at the neuromuscular junction, tendon cell development, and adhesion of wing epithelium.To address how Shot exerts its activity at the molecular level, we investigated the molecular interactions of Shot with candidate proteins in mature larval tendon cells. We show that Shot colocalizes with EB1/APC1 and with a compact microtubule array extending between the muscle-tendon junction and the cuticle. Shot forms a protein complex with EB1 via its C-terminal EF-hands and GAS2-containing domains. In tendon cells with reduced Shot activity, EB1/APC1 dissociate from the muscle-tendon junction, and the microtubule array elongates. The resulting tendon cell, although associated with the muscle and the cuticle ends, loses its stress resistance and elongates.Our results suggest that Shot mediates tendon stress resistance by the organization of a compact microtubule network at the muscle-tendon junction. This is achieved by Shot association with the cytoplasmic faces of the basal hemiadherens junction and with the EB1/APC1 complex.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (12)
	Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Act5C.PI Scer\GAL4^Bx-MS1096 Scer\GAL4^how-24B Scer\GAL4^sr-md710 shot^{APG.Scer\UAS.T:Avic\GFP} shot^{APT.Scer\UAS.T:Zzzz\TAP} shot^{dsRNA.Scer\UAS} shot^{EGG.Scer\UAS.T:Avic\GFP} shot^{EGT.Scer\UAS.T:Zzzz\TAP} shot^{PT.Scer\UAS.T:Zzzz\TAP} shot^SF20
Constructs (8)
	P{Act5C-GAL4} P{UAS-dskakRNA} P{UAS-mCD8::GFP.L} P{UAS-shot.APG} P{UAS-shot.APT} P{UAS-shot.EGG} P{UAS-shot.EGT} P{UAS-shot.PT}
Genes (14)
	Apc Apc2 Eb1 how Mmus\Cd8a mys Pax pbl Rho1 Scer\GAL4 shot sr T:Avic\GFP T:Zzzz\TAP
Insertions (3)
	P{GawB}Bx^MS1096 P{GawB}how^24B P{GawB}sr^md710
Natural transposons (1)
	P-element
Transcripts (2)
	Scer\GAL4^how-24BRA Scer\GAL4^sr-md710RA