Subject: GFP Protein Trap Insertion Lines
Personal communication from: Lynn Cooley, Yale School of Medicine,
Xavier Morin and William Chia, King's College London
To: Bloomington Drosophila Stock Center
Dated: October 2002 through May 2003
Subject: GFP Protein Trap Insertion Lines
Background: The information included here was provided by Lynn, Bill
or Xavier in connection with stocks donated to the stock center, or
it was extracted from http://flytrap.med.yale.edu/. These lines were
generated in the Chia lab, as described in Morin et al. 2001. Some
sequence data was generated in the Chia lab and some in the Cooley
lab. Expression patterns were determined by the Cooley group. Images
of the GFP expression patterns are at http://flytrap.med.yale.edu/.
Questions can be directed to Lynn Cooley and/or Bill Chia.
1. P{PTT-GC}ATPalphaG00109
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 93B2
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | membrane;
follicle cell | cytoplasm
2. P{PTT-GA}AsphZCL1605
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 52E4--5
viable: yes
fertile: yes
GFP expression pattern: follicle cell | cytoplasm (faint);
nurse cell | cytoplasm (faint)
3. P{PTT-GC}CG1640G00282
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 11F1
viable: yes
fertile: yes
GFP expression pattern: follicle cell | cytoplasm;
nurse cell | cytoplasm (punctate); nurse cell | nucleus;
oocyte | cytoplasm
4. P{PTT-GC}CG3735ZCL1614
sequence data:
GAACGTGCCTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGAC
GGGACCACCTTATGTTATTTCATCATGCCGAGTATCTTTCGCACACACG
TGTGGATCAACAAACATCTGGATTGGTGAAGGAATCCGATACACAGAC
ATATCGATAGCTCCTAGAGATGGCGTGTGGTGAATTTAAAGCCGGGAT
ATATTTTTTATATACATACTTTTCAAATCGCGCGCCCTCTTCATAATTC
ACCTCCACCACACCACGTTTCGTAGTTGCTCTTTCGCTGTCTCCCACCC
GCTCTCCGCAACACATTCACCTTTTGTTCGACGACCTTGGAGCGACTGT
CGTTAGTTCCGCGCGATTCGGTTCGCTCAAATGGTTCCGAGTGGTTCAT
TTCGTCTCAATAGAAATTAGTAATAAATATTTGTATGTACAATTTATTT
GCTCCAATATATTTGTATATATTTCCCTCACAGCTATATTTATTCTAATT
TAATATTATGACTTTTTAAGGTAATTTTTTGTGACCTGTTCGGAGTGAT
TAGCGTTACACNCTNNNNTCNGCTCCNCCCTCNANCGNNCN
estimated cytological location: 60A12
viable: yes
fertile: yes
GFP expression pattern: no ovary expression
5. P{PTT-GC}CG6084G00112
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 68C15
viable: yes
fertile: yes
GFP expression pattern: nurse cell | nucleus;
follicle cell | nucleus;
oocyte associated follicle cell | nucleus
6. P{PTT-GA}CG6416ZCL0663
sequence data:
ACACGAAATGAGAAACACACACAATTCGAACATACGATTGAACACGA
AAACCAGTCCATAGATGTTGTACGTACTACTTACTACTGCCTACTGCCG
GGATCACTCTCGGCATGGACGAGCTGTACAAGTCGACCTCGAGGTAAG
TTATTTGAACAATGGCATCAAATGCCTTCATCATCACTACCCTTTAGCC
CTTAAGACCCCACAATGACCTTACCCACTCAGAGAAAAAAGTAAATAT
GAAAGCCCATTTGAACTTCTCTGCAGCCAAGCTTTGCGTACTCGCAAA
TTATTAAAAATAAAACTTTAAAAATAATTTCGTCTAATTAATATTATGA
GTTAATTCAAACCCCACGGACCATTGCTAAGGANNNNNNNNNNNNNN
NNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNCNNNNNNNNTNNNN
NNNNNTNGNNNNNNNNGNNNNCNNNCGNNNNNNNNNNGGNNNNNNN
CCNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNCNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNN
NNNNCGGNNNNNNNNNNNNNNNNNNNNNNGNGNNNNNGCGNNCCNN
NNNNNCNNNNNNNNCCNNNNNNNNNNNNNNNCNNNNNNNNCNNNNN
CNNNNNNNNCNTNNNNNNCNNNNCNNNNCNCNNCNNNNNNNNNNNN
NNNNNNNNNNNCNCNNNCNNNNNNNNNNNNNGN
estimated cytological location: 66D8--9
viable: yes
fertile: yes
GFP expression pattern: ovarian sheath muscle
7. P{PTT-GB}CG8029ZCL0366
sequence data:
CTAGAAAAAAATTATTCGACTCTGAGAGCATTTCGTAAAGCCTAATTG
GATATTTGCACCAGGAACTGACATTAACTGGGTTTCTTTTCAAAAGTA
ATACTTATAAGAAAGTATAGNTTGTCAAATAAGGTTCATATCTTCGGT
GTCCAACTAGCGCAGTCACACACCTTTCCAGCTGGAAACTGTCAACGA
AACCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTCGACCTCGAG
GTAAGTTATTTGAACAATGGCATCAAATGCCTTCATCATCACTACCCTT
TAGCCCTTAAGACCCCACAATGACCTTACCCACTCAGAGAAAAAAGTA
AATATGAAAGCCCATTTGAACTTCTCTGCAGCCAAGCTTTGCGTACTC
GCAAATTATTAAAAATAAAACTTTAAAAATAATTTCGNCTAATTAATA
TTATGAGTTAATTCAAACCCCACGGACATGCTAAGGGGGNNCCCNNCC
CCCCCGGGNNGGGNNGCGGGNNGGCNGNCNCNNNGGGCNNCNCNCG
TCNGCCCCNNCNNNNGGCTNNCCCCGCCTGGGNTNCCGGCNCCACNCC
NGCCCCTGTNCCGNNCCCTTNNTCNTTGTCCCNNNNNNTNNCNCNGTC
CNCGNCNNNNCCGNNNNCNGGTNNNNCNNGNCCTNCNGCTCTNCGNN
NGTNNNNCCCNNTTNCCCCCCCNNCCGNCNTNNNTTNNCGTCNCCGCG
NNTGCCCNGGGCGTNCCNNCNCNTATGCGGTCCNCCCCCGCCNGNCTC
CCCTNNNTGCT
estimated cytological location: 45B4
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | nucleus;
nurse cell | nucleus
8. P{PTT-GA}CG8443G00271
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 52F6--7
viable: yes
fertile: yes
GFP expression pattern: follicle cell | cytoplasm;
nurse cell | cytoplasm (punctate); oocyte | cytoplasm
9. P{PTT-GC}CG10997G00264
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 12C2
viable: unknown
fertile: unknown
GFP expression pattern: nurse cell | cytoplasm
10. P{PTT-GA}CG14648ZCL3169
sequence data:
TGCTCATACGAATACATATTAATTGAGAAATTCGCGAGTCGATGCGCT
CATCGCCCAGAAATGTGTGCGTGCGAGTGTGTGCGTGTCGAAGCGTTG
CCAACTTATTGGTCATGCGTTAATATCGGGATATGGGTTCTTAATTTGT
ATTTCTCATTGAACCCGCCAAGAAAGACGGCCGAAATAGTTTTAGCGG
CCAATTCATACTTCAGTTTCAATTCAACAAGCGCACACACATGCATGTT
GACGTGTGTTCTTTAACCAGCACAGGTGGCAACCCTGCCGTCCCGATT
TTGCATGCAGATTTTTGTTTTCAATGATACGGGCCGGGATCACTCTCGG
CATGGACGAGCTGTACAAGTCGACTCGAGGTAATTATTTGAACAATGG
CATCAAATGCCTTCATCATCNACTACCCTTTAGCCCTTAAGACCCCACN
ATGACCTTACCCNACTCAGAGAAAAAAGTAAATATGAAAGCCCCATTT
GAACTTCCTCTGCAGCCAAGNTTTGCGTACTCGCTAATTATTAAAAAA
TAAAACTTTNAAGAATAATTTCCNTCTNAATTAAATATTATGAGTTAAT
TCAAACCCCCACNGCACNATGCCNAANGGATANTANTCCTCCCACNNC
TTNCTACCTNCCTNAACCNTACNCTCCCGNCGCATTNATNTCNTNTNCC
CGTNNCCCTANANGACCTATAGCCTGNCCTATNCTAANANAAAAAAN
NTNNN
estimated cytological location: 82B1
viable: yes
fertile: yes
GFP expression pattern: follicle cell | cytoplasm;
nurse cell | cytoplasm (punctate); oocyte | cytoplasm
11. P{PTT-GA}CG18763ZCL0931
sequence data:
GAACGTGNCTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGAC
GGGACCACCTTATGTTATTTCATCATGATGTGGCGCATCAAAACAAAA
ACAAACTACAGCTGATGCTGATTAGAGTTGGAGCCCGCTCTCTTCCAT
GCTCTCTCCCTCTCTCTATCTCTCACTTGTGGGGCAGAACCCAACTCGT
GGTGAGTTCTTCACTCCGCTTGTAGGTCACAAAAAAGTTTAAAATACG
CCAAGAACTGAAGCGAAAATAAAACATATACTTAAGCCGTGTTTAAG
ACACTTGCGGGATTAACATTCTGCATTTATTTATCTCATTTTATGTGCC
CCACCGGGATATATTTTTTATATACATACTTTTCAAATCGCGCGCCCTC
TTCATAATTCACCTCCACCACACCACGTTTCGTAGTTGCTCTTTCGCTG
TCTCCCACCCGCTCTCCGCAACACATTCACCTTTTGTTCGACGACCTTG
GAGCGACTGTCGTTAGTTCCGCGCGATTCGGTTCGCTCAAATGGTTCC
GAGTGGTTCATTTCGTCTCAATAGAAATTAGTAATAAATATTTGTATGT
ACAATTTATTTGCTCCAATATATTTGTATATATTTCCCTCACAGCTATA
TTTATTCTAATTTAATATTATGACTTTTTAAGGTAATTTTTTGTGACCTG
TTCGGAGTGATTTANCGTTACANCCNNNNNCNCGCCCGCNANAGNTTC
NCNCTTTTCCCCTGCCCNNCCCGTGNGTTATNTGGNNNGNCCCNNCGC
CC
estimated cytological location: 86E16
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | cytoplasm;
polar follicle cell; nurse cell | cytoplasm;
oocyte | cytoplasm
12. P{PTT-GB}CG30084G00189
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 52C4--6
viable: yes
fertile: yes
GFP expression pattern: ovarian sheath muscle
13. P{PTT-GA}CG31363G00147
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 86E16
viable: yes
fertile: yes
GFP expression pattern: follicle cell | cytoplasm;
follicle cell | membrane; border follicle cell;
polar follicle cell; nurse cell | cytoplasm;
nurse cell | nucleus; nurse cell | nuclear membrane;
oocyte | vitelline membrane; oocyte | cytoplasm
14. P{PTT-GC}Hrb98DEZCL0588
sequence data:
GCATTAGTAAAATGGTGAACTCGAACCAGAACCAGAACGGCAACTCC
AATGGCCATGATGATGTAAGTAGTGGATATTAAGGGTCTAGCAATTTC
CTGTAAACTGGTGTCTCGCCTTACCGGGATCACTCTCGGCATGGACGA
GCTGTACAAGTCGACACTCGAGGTAAGTTATTTGAACAATGGCATCAA
ATGCCTTCATCATCACTACCCTTTAGCCCTTAAGACCCCACAATGACCT
TACCCACTCAGAGAAAAAAGTAAATATGAAAGCCCATTTGAACTTCTC
TGCAGCCAAGCTTTGCGTACTCGCAAATTATTAAAAATAAAACTTTAA
AAATAATTTCGTCTAATTAATATTATGAGTTAATTCAAACCCCACGACC
ATGGCTTAAGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNGNNNNNGNNN
NNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNG
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNCCCN
NNNNNNCCNNNNNNNNNNNNNNNNCNNNNNNCNGNNNNNNGNNNNN
NCNNNNCNNCNNNCNCNNNNNNNCNNCCNNNCNNNNCNNNNNNNNN
NNNCCCCNCCNNNGNGNNNNNNNNNN
estimated cytological location: 98D6
viable: yes
fertile: yes
GFP expression pattern: follicle cell | nucleus;
nurse cell | nucleus
15. P{PTT-GB}IlkZCL3111
sequence data:
GAACGTGCCTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGAC
GGGACCACCTTATGTTATTTCATCATGATACGTATACTTATATATTTGA
TTCGTCTGTTTACACAACACAAGAATAAATCATGACGTCACTTAGACT
TGAAAGACCCTCGAAATCTTGGGCGTATTTAGGCGATGACTAAGTAAA
CGTTCCTGGTAGAGGGGGTTGCTACTTGAGGGCTTAACTTTCTAAGTG
CACTATAAAGCAACTAGAAAGTGATGGAGTTAGTTGCCTCTATTGGAT
TTACCCCAAATTGTTGTCGTGCTCCGTTTCATCCAACCAGAGGCGCACT
TGAATCGAGTTGCCCTCGCGGCACCAGTGGAATATGTCCTCCATTTTTA
CTCCTCACTCCTCTATTCCGCTCGCAGTCACTGATATTATAGGTTTTCC
AACCTAATTTACATCTAGAAACAGATTTAAACGCTTAAAATGCGACAG
TTGTGAGAATTCCAATTAAAATTTGTGCAGTGTGGACGTTTTAAATATT
TAACACACTGCTCGCGTTGCCAACTGCTGCAACACATCGACAAGTGTG
TATCGTGGTATCGATGACATACACCAATTGTGAATGTTTAAAGCAAGA
AGAAGAAGGAATACAACGTGCACCGCAACTATTATTTAAAAACATTGT
TGTTTACCTCATCTCTTTGTATTAATTAAACCATTACNAGCCCGAAAAT
GAAACTCAGCACATACAACTTCTTGACCTCCGTGGCCATCAAGGGCGT
CAAAGTGGG
estimated cytological location: 78C2
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | nucleus;
follicle cell | cytoplasm
16. P{PTT-un1}LacG00044
construct could be PTT-GA, PTT-GB or PTT-GC
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 49A6--7
viable: unknown (unverified lethal)
fertile: unknown
GFP expression pattern: no ovary expression
17. P{PTT-GB}LamCG00158
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 51B1
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | nucleus;
nurse cell | nucleus; oocyte | nucleus
18. P{PTT-GC}larpZCL2726
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 98C3
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | cytoplasm;
nurse cell | cytoplasm; oocyte | cytoplasm
19. P{PTT-GA}NrgG00305
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 7F3--4
viable: yes
fertile: yes
GFP expression pattern: follicle cell | cytoplasm;
follicle cell | membrane; border cell | membrane;
nurse cell | membrane (faint)
20. P{PTT-GA}Nrv2ZCL1649
sequence data:
GCACGTGCACTGAATTTAGTGTATACTTCGGTAAGCTTCGGCTATCGA
CGGGACCACCTTATGTTATTTCATCATGATTCAAACTATATACACTCGT
ATACATATACAGCAGTCCAGGTCCTTTTTGCTCTCTTAGTTTCTCTCAC
TTTTCACCCGACGTTTCAACCCCCCTGGCCGTTTTGGAAATTTGGCGCC
TTTTTCGGCTCTCCTGGCCGAAAAAAATGTTGCATCCGTCAGATACGA
CCTGCAACTGGTTGGCATGGAATGGGCTCGAAGGGCCAAACAATGTG
GGCCAAAATGGGGCCAGAAGGCCGTGCTACTGCTGGGGCGGCGACAT
GGTCCCATTTCCGCGTCTGCTGTTGTGATTTCAATGTCGCATTGGCTTT
GACCCGGGATATATTTTTTATATACATACTTTTCAAATCGCGCGCCCTC
TTCATAATTCACCTCCACCACACCACGTTTCGTAGTTGCTCTTTCGCTG
TCTCCCACCCGCTCTCCGCAACACATTCACCTTTTGTTCGACGACCTTG
GAGCGACTGTCGTTAGTTCCGCGCGATTCGGTTCGCTCAAATGGTTCC
GAGTGGTTCATTTCGTCTCAATAGAAATTAGTAATAAATATTTGTATGT
ACAATTTATTTGCTCCAATATATTTGTATATATTTCCCTCACAGCTATA
TTTATTCTAATTTAATATTATGACTTTTTAAGGTAATTTTTTGTGACCTG
TTCGGATGNAATAANACNGTTANAAANNNNNNNNNNNNNNNNNNNN
ANNNNNN
estimated cytological location: 27B1
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | cytoplasm (faint);
nurse cell | cytoplasm (faint); oocyte | cytoplasm (faint)
21. P{PTT-GA}PdiG00198
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 71B5
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | cytoplasm;
nurse cell | cytoplasm; oocyte | cytoplasm
22. P{PTT-GC}scyllaZCL0611
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 68B1--2
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | nucleus;
follicle cell | cytoplasm
23. P{PTT-GC}Tm2ZCL2456
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 88E12--13
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | cytoplasm
24. P{PTT-GA}VhaSFDG00259
sequence data: Morin et al. 2001 (FBrf0141725)
estimated cytological location: 36A6
viable: unknown
fertile: unknown
GFP expression pattern: follicle cell | cytoplasm;
follicle cell | membrane; nurse cell | cytoplasm;
oocyte | cytoplasm