Reference Report

Reference
Citation	Ainsley, J.A., Pettus, J.M., Bosenko, D., Gerstein, C.E., Zinkevich, N., Anderson, M.G., Adams, C.M., Welsh, M.J., Johnson, W.A. (2003). Enhanced locomotion caused by loss of the Drosophila DEG/ENaC protein Pickpocket1. Curr. Biol. 13(17): 1557--1563. (Export to RIS)
FlyBase ID	FBrf0162002
Publication Type	Research paper
PubMed ID	12956960
PubMed Abstract	Coordination of rhythmic locomotion depends upon a precisely balanced interplay between central and peripheral control mechanisms. Although poorly understood, peripheral proprioceptive mechanosensory input is thought to provide information about body position for moment-to-moment modifications of central mechanisms mediating rhythmic motor output. Pickpocket1 (PPK1) is a Drosophila subunit of the epithelial sodium channel (ENaC) family displaying limited expression in multiple dendritic (md) sensory neurons tiling the larval body wall and a small number of bipolar neurons in the upper brain. ppk1 null mutant larvae had normal external touch sensation and md neuron morphology but displayed striking alterations in crawling behavior. Loss of PPK1 function caused an increase in crawling speed and an unusual straight path with decreased stops and turns relative to wild-type. This enhanced locomotion resulted from sustained peristaltic contraction wave cycling at higher frequency with a significant decrease in pause period between contraction cycles. The mutant phenotype was rescued by a wild-type PPK1 transgene and duplicated by expressing a ppk1RNAi transgene or a dominant-negative PPK1 isoform. These results demonstrate that the PPK1 channel plays an essential role in controlling rhythmic locomotion and provide a powerful genetic model system for further analysis of central and peripheral control mechanisms and their role in movement disorders.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (2)
	Df(2L)A400 Df(2L)b88h49
Alleles (7)
	Avic\GFP^E.Scer\UAS Disc\RFP^Scer\UAS.cKa ppk^dsRNA.cAa ppk^{E145X.Scer\UAS} ppk^Scer\UAS.cAa Scer\GAL4^ppk.1.9 w^+mC
Constructs (6)
	P{ppk.RNAi} P{ppk-GAL4.1.9} P{UAS-AUG-DsRed} P{UAS-EGFP} P{UAS-ppk.A} P{UAS-ppk.E145X}
Genes (11)
	Avic\GFP Disc\RFP elA elB futsch noc pburs ppk Scer\GAL4 spel1 w
Transcripts (1)
	Scer\GAL4^ppk.1.9RA