Reference Report

Reference
Citation	Sasamura, T., Sasaki, N., Miyashita, F., Nakao, S., Ishikawa, H.O., Ito, M., Kitagawa, M., Harigaya, K., Spana, E., Bilder, D., Perrimon, N., Matsuno, K. (2003). neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions. Development 130(20): 4785--4795. (Export to RIS)
FlyBase ID	FBrf0162080
Publication Type	Research paper
PubMed ID	12917292
PubMed Abstract	Notch signalling, which is highly conserved from nematodes to mammals, plays crucial roles in many developmental processes. In the Drosophila embryo, deficiency in Notch signalling results in neural hyperplasia, commonly referred to as the neurogenic phenotype. We identify a novel maternal neurogenic gene, neurotic, and show that it is essential for Notch signalling. neurotic encodes a Drosophila homolog of mammalian GDP-fucose protein O-fucosyltransferase, which adds fucose sugar to epidermal growth factor-like repeats and is known to play a crucial role in Notch signalling. neurotic functions in a cell-autonomous manner, and genetic epistasis tests reveal that Neurotic is required for the activity of the full-length but not an activated form of Notch. Further, we show that neurotic is required for Fringe activity, which encodes a fucose-specific beta1, 3 N-acetylglucosaminyltransferase, previously shown to modulate Notch receptor activity. Finally, Neurotic is essential for the physical interaction of Notch with its ligand Delta, and for the ability of Fringe to modulate this interaction in Drosophila cultured cells. We present an unprecedented example of an absolute requirement of a protein glycosylation event for a ligand-receptor interaction. Our results suggest that O-fucosylation catalysed by Neurotic is also involved in the Fringe-independent activities of Notch and may provide a novel on-off mechanism that regulates ligand-receptor interactions.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (15)
	Avic\GFP^MtnA.PT Dl^{MtnA.T:Hsap\ALPP} fng^{MtnA.T:Ivir\HA1} fng^Scer\UAS.cKa N^ICN.Scer\UAS N^Scer\UAS.cMa O-fut1^4R6 O-fut1^{dsRNA.MtnA.cSa} O-fut1^{dsRNA.Scer\UAS.cSa} O-fut1^{G3.MtnA.T:Hsap\MYC} O-fut1^hs.PS O-fut1^{MtnA.T:Hsap\MYC} Scer\GAL4^ptc-559.1 Scer\GAL4^αTub84B.PL Scer\GAL80^αTub84B.PL
Constructs (6)
	P{hs-O-fut1.S} P{tubP-GAL4} P{tubP-GAL80} P{UAS-fng.K} P{UAS-N.ICN} P{UAS-O-fut1.dsRNA.S}
Genes (12)
	Avic\GFP Dl elav fng N O-fut1 Scer\GAL4 Scer\GAL80 T:Hsap\ALPP T:Hsap\MYC T:Ivir\HA1 wg
Insertions (1)
	P{GawB}ptc^559.1