Reference Report

Reference
Citation	Han, Y.G., Kwok, B.H., Kernan, M.J. (2003). Intraflagellar transport is required in Drosophila to differentiate sensory cilia but not sperm. Curr. Biol. 13(19): 1679--1686. (Export to RIS)
FlyBase ID	FBrf0167401
Publication Type	Research paper
PubMed ID	14521833
PubMed Abstract	Intraflagellar transport (IFT) uses kinesin II to carry a multiprotein particle to the tips of eukaryotic cilia and flagella and a nonaxonemal dynein to return it to the cell body. IFT particle proteins and motors are conserved in ciliated eukaryotes, and IFT-deficient mutants in algae, nematodes, and mammals fail to extend or maintain cilia and flagella, including sensory cilia. In Drosophila, the only ciliated cells are sensory neurons and sperm. no mechanoreceptor potential (nomp) mutations have been isolated that affect the differentiation and function of ciliated sense organs. The nompB gene is here shown to encode an IFT protein. Its mutant phenotypes reveal the consequences of an IFT defect in an insect.Mechanosensory and olfactory neurons in nompB mutants have missing or defective cilia. nompB encodes the Drosophila homolog of the IFT complex B protein IFT88/Polaris/OSM-5. nompB is expressed in the ciliated sensory neurons, and a functional, tagged NOMPB protein is located in sensory cilia and around basal bodies. Surprisingly, nompB mutant males produce normally elongated, motile sperm. Neuronally restricted expression and male germline mosaic experiments show that nompB-deficient sperm are fully functional in transfer, competition, and fertilization.NOMPB, the Drosophila homolog of IFT88, is required for the assembly of sensory cilia but not for the extension or function of the sperm flagellum. Assembly of this extremely long axoneme is therefore independent of IFT.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (1)
	Df(2L)03832MR1
Alleles (20)
	Avic\GFP^Scer\UAS.cUa Klp64D^k1 Klp64Dⁿ¹²³ l(2)03832a^03832a l(2)03832a^MR1 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} msl-2^{Hsp83.T:Ecol\dam,T:Hsap\MYC} nompB¹ nompB² nompB³ nompB^+tHa nompB^Scer\UAS.cHa nompB^{T:Avic\GFP-EGFP} nompB^unspecified nompB^ΔX.Scer\UAS Rfx⁴⁹ Rfx²⁵³ Scer\GAL4^elav-C155 Scer\GAL4^MJ94 T:Avic\GFP^EGFP
Constructs (7)
	P{EGFP-nompB} P{HDM} P{nompB⁺} P{UAS-GFP.U} P{UAS-mCD8::GFP.L} P{UAS-nompB.H} P{UAS-nompB.ΔX}
Genes (17)
	Ac3 Avic\GFP CG2201 Df31 futsch Klp64D l(2)03832a Lamp1 lin19 Mmus\Cd8a msl-2 nompB Rfx Scer\GAL4 T:Avic\GFP T:Ecol\dam T:Hsap\MYC
Insertions (3)
	P{GawB}elav^C155 P{GawB}MJ94 P{PZ}l(2)03832a^03832a
Natural transposons (1)
	Rt1b
Transcripts (1)
	Scer\GAL4^MJ94RA