Reference Report

Reference
Citation	Gaengel, K., Mlodzik, M. (2003). Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. Development 130(22): 5413--5423. (Export to RIS)
FlyBase ID	FBrf0167499
Publication Type	Research paper
PubMed ID	14507782
PubMed Abstract	Epidermal Growth Factor-receptor (Egfr) signaling is evolutionarily conserved and controls a variety of different cellular processes. In Drosophila these include proliferation, patterning, cell-fate determination, migration and survival. Here we provide evidence for a new role of Egfr signaling in controlling ommatidial rotation during planar cell polarity (PCP) establishment in the Drosophila eye. Although the signaling pathways involved in PCP establishment and photoreceptor cell-type specification are beginning to be unraveled, very little is known about the associated 90 degrees rotation process. One of the few rotation-specific mutations known is roulette (rlt) in which ommatidia rotate to a random degree, often more than 90 degrees. Here we show that rlt is a rotation-specific allele of the inhibitory Egfr ligand Argos and that modulation of Egfr activity shows defects in ommatidial rotation. Our data indicate that, beside the Raf/MAPK cascade, the Ras effector Canoe/AF6 acts downstream of Egfr/Ras and provides a link from Egfr to cytoskeletal elements in this developmentally regulated cell motility process. We provide further evidence for an involvement of cadherins and non-muscle myosin II as downstream components controlling rotation. In particular, the involvement of the cadherin Flamingo, a PCP gene, downstream of Egfr signaling provides the first link between PCP establishment and the Egfr pathway.
DOI
Related Publication(s)
Erratum	Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. Gaengel and Mlodzik, 2003, Development 130(23): 5861 [FBrf0178916]
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (10)
	Df(3L)81k19 Df(3L)Cat Df(3L)D-5rv12 Df(3L)st7 Df(3L)st7P Df(3L)st-f13 Df(3L)st-j7 Df(3L)th102 Df(3L)W10 In(3L)std11
Alleles (50)
	aos^rlt aos^sev.PF aos^W11 aos^Δ7 arm² btl::Egfr^{Scer\UAS.T:λ\cI-DD} capt⁰⁶⁹⁵⁵ chic⁰¹³²⁰ ck¹³ cno² cno³ cno^mis1 cno^Scer\UAS.cMa Ecol\lacZ^HLHmδ.0.5 Ecol\lacZ^svp-07842 Egfr^t1 Egfr^top-EC20 ena⁰²⁰²⁹ grk³ Hsap\RALA^G23V.GMR Khc⁸ Khc^k13314 kst⁰¹³¹⁸ mys¹ mys^unspecified nmo^P1 Pi3K92E^A Pi3K92E^{Scer\UAS.T:Hsap\MYC} pnt^Δ88 pnut⁰²⁵⁰² pnut^XP Ras85D^e2F Ras85D^{V12.C40.Scer\UAS} Ras85D^{V12.G37.Scer\UAS} Ras85D^{V12.S35.Scer\UAS} Ras85D^V12.Scer\UAS S^48-5 sca^BP1 Scer\GAL4^HLHmδ.0.5 Scer\GAL4^hs.2sev shg² spi¹ spi²⁵⁹ stan^E59 T:λ\cI^DD vn^addC6 wb⁴⁴¹⁸ wb⁰⁹⁴³⁷ zip¹ zip⁰²⁹⁵⁷
Constructs (11)
	P{GAL4-Hsp70.sev} P{HLHmδ-lacZ.0.5} P{mδ0.5-GAL4} P{Sev-argos} P{UAS-cno} P{UAS-Egfr.λtop} P{UAS-Ras85D.V12.C40} P{UAS-Ras85D.V12.G37} P{UAS-Ras85D.V12.S35} P{UAS-Ras85D.V12} P{WT-Dp110}
Genes (36)
	aos arm B boss btl::Egfr capt chic ck cno E(spl)mδ-HLH Ecol\lacZ Egfr elav ena grk Hsap\RALA Khc kst mys nmo Pi3K92E pnt pnut Ras85D S sca Scer\GAL4 shg spi stan svp T:Hsap\MYC T:λ\cI vn wb zip
Insertions (1)
	P{PZ}svp⁰⁷⁸⁴²