Reference Report

Reference
Citation	Lee, A., Li, W., Xu, K., Bogert, B.A., Su, K., Gao, F.B. (2003). Control of dendritic development by the Drosophila fragile X-related gene involves the small GTPase Rac1. Development 130(22): 5543--5552. (Export to RIS)
FlyBase ID	FBrf0167501
Publication Type	Research paper
PubMed ID	14530299
PubMed Abstract	Fragile X syndrome is caused by loss-of-function mutations in the fragile X mental retardation 1 gene. How these mutations affect neuronal development and function remains largely elusive. We generated specific point mutations or small deletions in the Drosophila fragile X-related (Fmr1) gene and examined the roles of Fmr1 in dendritic development of dendritic arborization (DA) neurons in Drosophila larvae. We found that Fmr1 could be detected in the cell bodies and proximal dendrites of DA neurons and that Fmr1 loss-of-function mutations increased the number of higher-order dendritic branches. Conversely, overexpression of Fmr1 in DA neurons dramatically decreased dendritic branching. In dissecting the mechanisms underlying Fmr1 function in dendrite development, we found that the mRNA encoding small GTPase Rac1 was present in the Fmr1-messenger ribonucleoprotein complexes in vivo. Mosaic analysis with a repressor cell marker (MARCM) and overexpression studies revealed that Rac1 has a cell-autonomous function in promoting dendritic branching of DA neurons. Furthermore, Fmr1 and Rac1 genetically interact with each other in controlling the formation of fine dendritic branches. These findings demonstrate that Fmr1 affects dendritic development and that Rac1 is partially responsible for mediating this effect.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (14)
	Fmr1¹ Fmr1^2.L Fmr1³ Fmr1^4.L Fmr1^+t14 Fmr1^EP3517 Fmr1^Scer\UAS.cWa Fmr1^{Scer\UAS.T:Avic\GFP} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rac1^J11 Rac1^{Scer\UAS.T:Hsap\MYC} Scer\GAL4^109(2)80 Scer\GAL4^elav-C155 Scer\GAL4^αTub84B.PL
Constructs (6)
	P{Fmr1.14} P{tubP-GAL4} P{UAS-Fmr1.GFP} P{UAS-Fmr1.W} P{UAS-mCD8::GFP.L} P{UAS-Rac1.Myc}
Genes (8)
	Fmr1 Hk Mmus\Cd8a Rac1 Scer\GAL4 T:Avic\GFP T:Hsap\MYC αTub84B
Insertions (2)
	P{GawB}109(2)80 P{GawB}elav^C155