Reference Report

Reference
Citation	Estella, C., Rieckhof, G., Calleja, M., Morata, G. (2003). The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila. Development 130(24): 5929--5941. (Export to RIS)
FlyBase ID	FBrf0167505
Publication Type	Research paper
PubMed ID	14561634
PubMed Abstract	The related genes buttonhead (btd) and Drosophila Sp1 (the Drosophila homologue of the human SP1 gene) encode zinc-finger transcription factors known to play a developmental role in the formation of the Drosophila head segments and the mechanosensory larval organs. We report a novel function of btd and Sp1: they induce the formation and are required for the growth of the ventral imaginal discs. They act as activators of the headcase (hdc) and Distal-less (Dll) genes, which allocate the cells of the disc primordia. The requirement for btd and Sp1 persists during the development of ventral discs: inactivation by RNA interference results in a strong reduction of the size of legs and antennae. Ectopic expression of btd in the dorsal imaginal discs (eyes, wings and halteres) results in the formation of the corresponding ventral structures (antennae and legs). However, these structures are not patterned by the morphogenetic signals present in the dorsal discs; the cells expressing btd generate their own signalling system, including the establishment of a sharp boundary of engrailed expression, and the local activation of the wingless and decapentaplegic genes. Thus, the Btd product has the capacity to induce the activity of the entire genetic network necessary for ventral imaginal discs development. We propose that this property is a reflection of the initial function of the btd/Sp1 genes that consists of establishing the fate of the ventral disc primordia and determining their pattern and growth.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(1)C52 Dp(1;Y)lz⁺
Alleles (21)
	btd^{dsRNA.Scer\UAS} btd^MD808 btd^Scer\UAS.cSa btd^unspecified btd^XG Dll¹⁷ Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^esg-6 Ecol\lacZ^hdc-B5 Ecol\lacZ^wg-en11 Scer\GAL4⁴⁴⁴ Scer\GAL4^Act5C.PP Scer\GAL4^bi-omb-Gal4 Scer\GAL4^btd-MD808 Scer\GAL4^Dll-MD743 Scer\GAL4^ey.PH Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^nub-AC-62 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Sp1^{dsRNA.Scer\UAS} Ubx¹
Constructs (8)
	P{BS3.0} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{GAL4-ey.H} P{matα4-GAL-VP16} P{UAS-btd.S} P{UAS-btd-RNAi} P{UAS-Sp1-RNAi}
Genes (15)
	btd dac Dll dpp Ecol\lacZ en esg hdc hth Scer\GAL4 Sp1 T:Hsim\VP16 Ubx vg wg
Insertions (9)
	Doc{}Ubx¹ P{en1}wg^en11 P{enG}6 P{GAL4}bi^omb-Gal4 P{GAL4}MD743 P{GawB}444 P{GawB}btd^MD808 P{GawB}nubbin-AC-62 P{lacZ-un1}hdc^B5
Transcripts (3)
	Scer\GAL4⁴⁴⁴RA Scer\GAL4^btd-MD808RA Scer\GAL4^nub-AC-62RA