Reference Report

Reference
Citation	Desbordes, S.C., Sanson, B. (2003). The glypican Dally-like is required for Hedgehog signalling in the embryonic epidermis of Drosophila. Development 130(25): 6245--6255. (Export to RIS)
FlyBase ID	FBrf0167512
Publication Type	Research paper
PubMed ID	14602684
PubMed Abstract	The Drosophila genes dally and dally-like encode glypicans, which are heparan sulphate proteoglycans anchored to the cell membrane by a glycosylphosphatidylinositol link. Genetic studies have implicated Dally and Dally-like in Wingless signalling in embryos and imaginal discs. Here, we test the signalling properties of these molecules in the embryonic epidermis. We demonstrate that RNA interference silencing of dally-like, but not dally, gives a segment polarity phenotype identical to that of null mutations in wingless or hedgehog. Using heterologous expression in embryos, we uncoupled the Hedgehog and Wingless signalling pathways and found that Dally-like and Dally, separately or together, are not necessary for Wingless signalling. Dally-like, however, is strictly necessary for Hedgehog signal transduction. Epistatic experiments show that Dally-like is required for the reception of the Hedgehog signal, upstream or at the level of the Patched receptor.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (19)
	dally^dsRNA.3' dally^dsRNA.5' dally^dsRNA.mid dlp^dsRNA.3' dlp^dsRNA.5' dlp^Scer\UAS.cBa Ecol\lacZ^NUC.Scer\UAS hh^AC hh^N.Scer\UAS hh^Scer\UAS.cIa ptc¹⁶ Scer\GAL4^arm.PS Scer\GAL4^{arm.T:Hsim\VP16} Scer\GAL4^en-e16E Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.arm.T:Hsim\VP16} Scer\GAL4^sim.PS wg^l-17 wg^Scer\UAS.cLa
Constructs (9)
	P{GAL4-arm.S} P{GAL4-arm(FRT.y⁺).VP16} P{GAL4-arm(-FRT).VP16} P{GAL4-sim.S} P{UAS-dlp.WT} P{UAS-hh.I} P{UAS-hh.N} P{UAS-lacZ.NUC} P{UAS-wg.L}
Genes (10)
	dally dlp Ecol\lacZ en hh ptc rho Scer\GAL4 T:Hsim\VP16 wg
Insertions (2)
	P{en2.4-GAL4}e16E P{GawB}ptc^559.1