Reference Report

Reference
Citation	Carrera, P., Moshkin, Y.M., Gronke, S., Sillje, H.H., Nigg, E.A., Jackle, H., Karch, F. (2003). Tousled-like kinase functions with the chromatin assembly pathway regulating nuclear divisions. Genes Dev. 17(20): 2578--2590. (Export to RIS)
FlyBase ID	FBrf0167605
Publication Type	Research paper
External Crossreferences
PubMed ID	14561777
PubMed Abstract	Tousled-like kinases (TLKs) constitute a family of serine/threonine kinases conserved in plants and animals that act in a cell cycle-dependent manner. In mammals, their activity peaks during S phase, when they phosphorylate the antisilencing function protein 1 (ASF1), a histone chaperone involved in replication-dependent chromatin assembly. Here, we show that Drosophila ASF1 is also a phosphorylation target of TLK, and that the two components cooperate to control chromatin replication in vivo. By altering TLK activity through loss-of-function mutations, we show that nuclear divisions are arrested at interphase, followed by apoptosis. Overexpression of TLK alters the chromatin structure, suggesting that TLK mediates the activity of chromatin proteins. These results suggest that TLK coordinates cell cycle progression through the regulation of chromatin dynamics.
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Editors
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (11)
	asf1^Scer\UAS.cMa Scer\GAL4^arm.PS Scer\GAL4^Bx-MS1096 Scer\GAL4^ey.PH Scer\GAL4^hs.2sev Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} tlk^{dead.Scer\UAS} tlk^G0054 tlk^Scer\UAS.cCa tlk^Δ14 tlk^Δ40
Constructs (7)
	P{GAL4-arm.S} P{GAL4-ey.H} P{GAL4-Hsp70.sev} P{matα4-GAL-VP16} P{UAS-asf1.M} P{UAS-tlk.C} P{UAS-tlk.dead}
Genes (6)
	asf1 His3 Lam Scer\GAL4 T:Hsim\VP16 tlk
Insertions (2)
	P{GawB}Bx^MS1096 P{lacW}tlk^G0054
Polypeptides (1)
	tlk-XP
Transcripts (1)
	tlk-XR