Reference Report

Reference
Citation	Jia, J., Tong, C., Jiang, J. (2003). Smoothened transduces Hedgehog signal by physically interacting with Costal2/Fused complex through its C-terminal tail. Genes Dev. 17(21): 2709--2720. (Export to RIS)
FlyBase ID	FBrf0167606
Publication Type	Research paper
PubMed ID	14597665
PubMed Abstract	The Hedgehog (Hh) family of secreted proteins controls many aspects of growth and patterning in animal development. The seven-transmembrane protein Smoothened (Smo) transduces the Hh signal in both vertebrates and invertebrates; however, the mechanism of its action remains unknown. We found that Smo lacking its C-terminal tail (C-tail) is inactive, whereas membrane-tethered Smo C-tail has constitutive albeit low levels of Hh signaling activity. Smo physically interacts with Costal2 (Cos2) and Fused (Fu) through its C-tail. Deletion of the Cos2/Fu-binding domain from Smo abolishes its signaling activity. Moreover, overexpressing Cos2 mutants that fail to bind Fu and Ci but retain Smo-binding activity blocks Hh signaling. Taken together, our results suggest that Smo transduces the Hh signal by physically interacting with the Cos2/Fu protein complex.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (33)
	cos^{CC.Scer\UAS.T:Ivir\HA1} cos^{CT1.Scer\UAS.T:Ivir\HA1} cos^{CT2.Scer\UAS.T:Ivir\HA1} cos^{MB.Scer\UAS.T:Ivir\HA1} cos^{Scer\UAS.T:Ivir\HA1} cos^{ΔC.Scer\UAS.T:Ivir\HA1} cos^{ΔN1.Scer\UAS.T:Ivir\HA1} cos^{ΔN2.Scer\UAS.T:Ivir\HA1} Ecol\lacZ^dpp.BS3.0 hh^Scer\UAS.cAa ptc^Scer\UAS.cJa Scer\GAL4^Act5C.PP Scer\GAL4^Bx-MS1096 Scer\GAL4^prd.RG1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL4^unspecified smo³ smo^{CT.556-730.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{CT.556-818.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{CT.730-818.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{CT.730-1035.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{CT.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{CT.Scer\UAS.T:Zzzz\FLAG,T:TM-sev} smo^{CT.Scer\UAS.T:Zzzz\FLAG} smo^{CT.Δ625-730.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{CT.Δ625-818.Scer\UAS.T:Zzzz\FLAG,T:Myr-Src64B} smo^{Scer\UAS.J.T:Avic\GFP} smo^{Scer\UAS.T:Hsap\MYC} smo^{ΔC730.Scer\UAS.T:Avic\GFP} smo^{ΔC818.Scer\UAS.T:Avic\GFP} smo^{ΔCT.Scer\UAS.T:Avic\GFP} smo^{ΔCT.Scer\UAS.T:Hsap\MYC} Su(fu)^LP
Constructs (29)
	P{BS3.0} P{GAL4} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-Act5C(-FRT).P} P{UAS-Cos2.CC.HA} P{UAS-Cos2.CT1.HA} P{UAS-Cos2.CT2.HA} P{UAS-Cos2.MB.HA} P{UAS-Cos2.W} P{UAS-Cos2.ΔC.HA} P{UAS-Cos2.ΔN1.HA} P{UAS-Cos2.ΔN2.HA} P{UAS-hh.A} P{UAS-ptc.J} P{UAS-smo.CT.556-730.Myr} P{UAS-smo.CT.556-818.Myr} P{UAS-smo.CT.730-818.Myr} P{UAS-smo.CT.730-1035.Myr} P{UAS-smo.CT.Flag} P{UAS-smo.CT.Myr} P{UAS-smo.CT.Sev} P{UAS-smo.CT.Δ625-730.Myr} P{UAS-smo.CT.Δ625-818.Myr} P{UAS-smo.GFP} P{UAS-smo.Myc} P{UAS-smo.ΔC730.GFP} P{UAS-smo.ΔC818.GFP} P{UAS-smo.ΔCT.GFP} P{UAS-smo.ΔCT.Myc}
Genes (15)
	ci cos Ecol\lacZ fu hh ptc Scer\GAL4 smo Su(fu) T:Avic\GFP T:Hsap\MYC T:Ivir\HA1 T:Myr-Src64B T:TM-sev T:Zzzz\FLAG
Insertions (1)
	P{GawB}Bx^MS1096
Natural transposons (1)
	P-element