Reference Report

Reference
Citation	Dammai, V., Adryan, B., Lavenburg, K.R., Hsu, T. (2003). Drosophila awd, the homolog of human nm23, regulates FGF receptor levels and functions synergistically with shi/dynamin during tracheal development. Genes Dev. 17(22): 2812--2824. (Export to RIS)
FlyBase ID	FBrf0167607
Publication Type	Research paper
PubMed ID	14630942
PubMed Abstract	Human nm23 has been implicated in suppression of metastasis in various cancers, but the underlying mechanism of such activity has not been fully understood. Using Drosophila tracheal system as a genetic model, we examined the function of the Drosophila homolog of nm23, the awd gene, in cell migration. We show that loss of Drosophila awd results in dysregulated tracheal cell motility. This phenotype can be suppressed by reducing the dosage of the chemotactic FGF receptor (FGFR) homolog, breathless (btl), indicating that btl and awd are functionally antagonists. In addition, mutants of shi/dynamin show similar tracheal phenotypes as in awd and exacerbate those in awd mutant, suggesting defects in vesicle-mediated turnover of FGFR in the awd mutant. Consistent with this, Btl-GFP chimera expressed from a cognate btl promoter-driven system accumulate at high levels on tracheal cell membrane of awd mutants as well as in awd RNA duplex-treated cultured cells. Thus, we propose that awd regulates tracheal cell motility by modulating the FGFR levels, through a dynamin-mediated pathway.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (1)
	Df(3R)awd-KRB
Alleles (12)
	awd^dsRNA.cPa awd^j2A4 awd^KRN3 awd^Scer\UAS.cDa btl^H82Δ3 btl^{MtnA.T:Avic\GFP-S65T} btl^{Scer\UAS.T:Avic\GFP-S65T} Ecol\lacZ^trh-1-eve-1 Egfr^2.MtnA Scer\GAL4^btl.PS shi¹ T:Avic\GFP^S65T
Constructs (3)
	P{GAL4-btl.S} P{UAS-awd.D} P{UAS-btl::GFP-S65T}
Genes (10)
	awd btl Dl Ecol\lacZ Egfr N Scer\GAL4 shi T:Avic\GFP trh
Insertions (3)
	P{5'lwB}btl^H82Δ3 P{ET-L}trh-1-eve-1 P{lacW}awd^j2A4