Reference Report

Reference
Citation	Greil, F., van der Kraan, I., Delrow, J., Smothers, J.F., de Wit, E., Bussemaker, H.J., van Driel, R., Henikoff, S., van Steensel, B. (2003). Distinct HP1 and Su(var)3-9 complexes bind to sets of developmentally coexpressed genes depending on chromosomal location. Genes Dev. 17(22): 2825--2838. (Export to RIS)
FlyBase ID	FBrf0167608
Publication Type	Research paper
PubMed ID	14630943
PubMed Abstract	Heterochromatin proteins are thought to play key roles in chromatin structure and gene regulation, yet very few genes have been identified that are regulated by these proteins. We performed large-scale mapping and analysis of in vivo target loci of the proteins HP1, HP1c, and Su(var)3-9 in Drosophila Kc cells, which are of embryonic origin. For each protein, we identified approximately 100-200 target genes among >6000 probed loci. We found that HP1 and Su(var)3-9 bind together to transposable elements and genes that are predominantly pericentric. In addition, Su(var)3-9 binds without HP1 to a distinct set of nonpericentric genes. On chromosome 4, HP1 binds to many genes, mostly independent of Su(var)3-9. The binding pattern of HP1c is largely different from those of HP1 and Su(var)3-9. Target genes of HP1 and Su(var)3-9 show lower expression levels in Kc cells than do nontarget genes, but not if they are located in pericentric regions. Strikingly, in pericentric regions, target genes of Su(var)3-9 and HP1 are predominantly embryo-specific genes, whereas on the chromosome arms Su(var)3-9 is preferentially associated with a set of male-specific genes. These results demonstrate that, depending on chromosomal location, the HP1 and Su(var)3-9 proteins form different complexes that associate with specific sets of developmentally coexpressed genes.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (5)
	HP1c^{hs.T:Ecol\dam,T:Hsap\MYC} Su(var)3-9^dsRNA.cGa Su(var)3-9^{hs.T:Ecol\dam,T:Hsap\MYC} Su(var)205^dsRNA.cGa Su(var)205^{hs.T:Ecol\dam,T:Hsap\MYC}
Genes (116)
	Ac3 Ald Ank ApepP Arp2 Best1 bip2 blot Cals CaMKII Camta Cdk4 chp Cht3 cin ck CkIIα CkIIα-i3 cno Cp1 Crk Cyp28d1 d4 Dcr-1 Df31 Dhc64C dhd disp DNApol-α180 dos Ect4 edl eIF4G Ephrin ERR fal GATAd Gel Glt glu Hex-A His1 His2A His2B His3 His4 HP1b HP1c Iap2 lgs lilli Lrr47 Lsd-2 mdy Men Mlc2 mnd mRpS7 msps mthl8 mud myo NaCP60E nAcRα-80B nej Nipped-A Nipped-B p120ctn Pfrx PGRP-LB Plc21C poe Prx3 Psi puc pug Pur-α pyd3 regucalcin Rgl Rh50 RpII215 rpk SamDC sano scrib scyl sec6 sls Sod2 Spp Srp54 Strn-Mlck Su(var)3-9 Su(var)205 Surf6 sxc T:Ecol\dam T:Hsap\MYC Taf12 Tif-IA tio tok toy tra2 trx Tsp42Ei tws U2af50 unc-13 wus XRCC1 zfh2 α-Cat γSnap δCOP
Natural transposons (29)
	17.6 297 1360 1731 baggins copia Cr1a Doc Doc3-element F-element flea gypsy5 HMS-Beagle hobo I-element INE-1 invader4 Ivk Max-element McClintock opus R1-element roo springer Stalker2 Tc1 Tirant Tom1 X-element