Reference Report

Reference
Citation	Fouix, S., Martin-Lanneree, S., Sanial, M., Morla, L., Lamour-Isnard, C., Plessis, A. (2003). Over-expression of a novel nuclear interactor of Suppressor of fused, the Drosophila myelodysplasia/myeloid leukaemia factor, induces abnormal morphogenesis associated with increased apoptosis and DNA synthesis. Genes Cells 8(11): 897--911. (Export to RIS)
FlyBase ID	FBrf0167614
Publication Type	Research paper
PubMed ID	14622141
PubMed Abstract	In Drosophila and vertebrates, suppressor of fused (Su(fu)) proteins act as negative regulators of the Gli/Ci transcription factors, which mediate the transcriptional effects of Hh signalling.We sought for novel partners of Su(fu) in fly using the two-hybrid method. Most of the Su(fu) interactors thus identified are (or are likely to be) able to enter the nucleus. We focused on one of these putative partners, dMLF, which resembles vertebrate myelodysplasia/myeloid leukaemia factors 1 and 2. We demonstrate that dMLF binds specifically to Su(fu) in vitro and in vivo. Using a novel anti-dMLF antibody, we showed, that dMLF is a nuclear, chromosome-associated protein. We over-expressed a dMLF transgene in fly using an inducible expression system and showed that dMLF over-expression disrupts normal development, leading to either a lethal phenotype or adult structural defects associated with apoptosis and increased DNA synthesis. Furthermore, the dMLF-induced eye phenotype is enhanced by the loss of Su(fu) function, suggesting a genetic interaction between Su(fu) and dMLF.We propose that dSu(fu) and dMLF act together at the transcriptional level to coordinate patterning and proliferation during development.
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Cells
Title	Genes to cells : devoted to molecular & cellular mechanisms
Publication Year	1996-
ISBN/ISSN	1356-9597
Data from Reference
Alleles (10)
	Mlf^Scer\UAS.cFa Scer\GAL4^Bx-MS1096 Scer\GAL4^da.G32 Scer\GAL4^en-e16E Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^ptc-559.1 Scer\GAL4^vg.PM Su(fu)^LP Su(fu)^Scer\UAS.cDa
Constructs (6)
	P{GAL4-da.G32} P{GAL4-ey.H} P{GAL4-ninaE.GMR} P{GAL4-vg.M} P{UAS-Mlf.F} P{UAS-Su(fu).D}
Genes (16)
	bcd Cenp-C CG4364 CG7922 cos Ef1β fu Mi-2 Mlf mtrm osk RpL6 Rpt2 Scer\GAL4 Su(fu) Trf4-1
Insertions (7)
	P{en2.4-GAL4}e16E P{GawB}Bx^MS1096 P{GawB}ptc^559.1 P{UAS-Mlf.F}21 P{UAS-Mlf.F}47 P{UAS-Mlf.F}69 P{UAS-Mlf.F}77
Natural transposons (1)
	P-element