Reference Report

Reference
Citation	Bayer, C.A., Halsell, S.R., Fristrom, J.W., Kiehart, D.P., von Kalm, L. (2003). Genetic interactions between the RhoA and Stubble-stubbloid loci suggest a role for a type II transmembrane serine protease in intracellular signaling during Drosophila imaginal disc morphogenesis. Genetics 165(3): 1417--1432. (Export to RIS)
FlyBase ID	FBrf0167640
Publication Type	Research paper
PubMed ID	14668391
PubMed Abstract	The Drosophila RhoA (Rho1) GTPase is essential for postembryonic morphogenesis of leg and wing imaginal discs. Mutations in RhoA enhance leg and wing defects associated with mutations in zipper, the gene encoding the heavy chain of nonmuscle myosin II. We demonstrate here that mutations affecting the RhoA signaling pathway also interact genetically with mutations in the Stubble-stubbloid (Sb-sbd) locus that encodes an unusual type II transmembrane serine protease required for normal leg and wing morphogenesis. In addition, a leg malformation phenotype associated with overexpression of Sb-sbd in prepupal leg discs is suppressed when RhoA gene dose is reduced, suggesting that RhoA and Sb-sbd act in a common pathway during leg morphogenesis. We also characterized six mutations identified as enhancers of zipper mutant leg defects. Three of these genes encode known members of the RhoA signaling pathway (RhoA, DRhoGEF2, and zipper). The remaining three enhancer of zipper mutations interact genetically with both RhoA and Sb-sbd mutations, suggesting that they encode additional components of the RhoA signaling pathway in imaginal discs. Our results provide evidence that the type II transmembrane serine proteases, a class of proteins linked to human developmental abnormalities and pathology, may be associated with intracellular signaling required for normal development.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (6)
	Df(2R)ES1 Df(2R)Jp1 Df(2R)Jp4 Df(2R)Jp8 Df(2R)P803-Δ15 Df(3R)sbd105
Alleles (41)
	bl^unspecified br¹ bs^unspecified cora^unspecified crol^unspecified cta^unspecified E(zip)12-5^12-5 E(zip)18-5^18-5 E(zip)31-6^31-6 ex^unspecified insc^unspecified Pkn³ Pkn⁰⁶⁷³⁶ Pkn^unspecified Rho1^12-6 Rho1^72F Rho1^72O Rho1^E3.10 Rho1^J3.8 RhoGEF2^1.1 RhoGEF2^4.1 RhoGEF2^11-3 RhoGEF2⁰⁴²⁹¹ rok¹ rok² Sb¹ Sb^63b Sb⁷⁰ Sb^hs.PB Sb^sbd-1 Sb^sbd-2 Sb^sbd-201 Sb^Spi Sb^unspecified sqh¹ sqh² sqh^unspecified vlc^unspecified w^+mC zip^33-1 zip^Ebr
Constructs (1)
	P{hs-Sb.B}
Genes (20)
	bl br bs cora crol cta E(zip)12-5 E(zip)18-5 E(zip)31-6 ex insc Pkn Rho1 RhoGEF2 rok Sb sqh vlc w zip