Open Close
Reference
Citation
Clyne, P.J., Brotman, J.S., Sweeney, S.T., Davis, G. (2003). Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements.  Genetics 165(3): 1433--1441.
FlyBase ID
FBrf0167641
Publication Type
Research paper
Abstract

We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This technique uses a new, small P transposable element (the Wee-P) that is composed primarily of the green fluorescent protein (GFP) sequence flanked by consensus splice acceptor and splice donor sequences. We demonstrate that insertion of the Wee-P can generate GFP fusions with native proteins. We further demonstrate that GFP-tagged proteins have correct subcellular localization and can be expressed at near-normal levels. We have used the Wee-P to tag genes with a wide variety of functions, including transmembrane proteins. A genetic analysis of 12 representative fusion lines demonstrates that loss-of-function phenotypes are not caused by the Wee-P insertion. This technology allows the generation of GFP-tagged reagents on a genome-wide scale with diverse potential applications.

PubMed ID
PubMed Central ID
PMC1462835 (PMC) (EuropePMC)
DOI
Related Publication(s)
Erratum

Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements.
Clyne et al., 2004, Genetics 167(4): 2143 [FBrf0179796]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference